• Western blot analysis of various lysates using NUBP2 Rabbit polyclonal antibody (STJ118904) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-NUBP2 antibody (142-271) (STJ118904)

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STJ118904

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NUBP2 (142-271) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NUBP2
Gene ID: 10101
Uniprot ID: NUBP2_HUMAN
Immunogen Region: 142-271
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 142-271 of human NUBP2 (NP_036357.1).
Immunogen Sequence: MATIEALRPYQPLGALVVTT PQAVSVGDVRRELTFCRKTG LRVMGIVENMSGFTCPHCTE CTSVFSRGGGEELAQLAGVP FLGSVPLDPALMRTLEEGHD FIQEFPGSPAFAALTSIAQK ILDATPACLP
Tissue Specificity Widely expressed with highest expression in skeletal muscle.
Function Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Negatively regulates cilium formation and structure.
Protein Name Cytosolic Fe-S Cluster Assembly Factor Nubp2
Nucleotide-Binding Protein 2
Nbp 2
Database Links Reactome: R-HSA-2564830
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Cilium Axoneme
Centriole
Enriched At The Centrosomes During Mitosis
Enriched In Centrioles Of Microtubule Asters During Prophase
Prometaphase And Telophase Stages Of Mitosis
Localized At Centrioles And In The Nucleus At Interphase
Colocalizes With Nubp-1 At Prometaphase
Alternative Antibody Names Anti-Cytosolic Fe-S Cluster Assembly Factor Nubp2 antibody
Anti-Nucleotide-Binding Protein 2 antibody
Anti-Nbp 2 antibody
Anti-NUBP2 antibody

Information sourced from Uniprot.org

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