Western blot analysis of various lysates using NUBP1 Rabbit polyclonal antibody (STJ118832) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-NUBP1 antibody (201-320) (STJ118832)

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STJ118832
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NUBP1
Gene ID: 4682
Uniprot ID: NUBP1_HUMAN
Immunogen Region: 201-320
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 201-320 of human NUBP1 (NP_002475.2).
Immunogen Sequence: PQEVSLQDVRKEINFCRKVK LPIIGVVENMSGFICPKCKK ESQIFPPTTGGAELMCQDLE VPLLGRVPLDPLIGKNCDKG QSFFIDAPDSPATLAYRSII QRIQEFCNLHQSKEENLISS
Function Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Implicated in the regulation of centrosome duplication. Negatively regulates cilium formation and structure.
Protein Name Cytosolic Fe-S Cluster Assembly Factor Nubp1
Nucleotide-Binding Protein 1
Nbp 1
Database Links Reactome: R-HSA-2564830
Cellular Localisation Cytoplasm
Nucleus
Cell Projection
Cytoskeleton
Cilium Axoneme
Cilium Basal Body
Microtubule Organizing Center
Centrosome
Centriole
Enriched In Centrioles Of Microtubule Asters During Prophase
Prometaphase And Telophase Stages Of Mitosis
Localized At Centrioles And In The Nucleus At Interphase
Colocalizes With Nubp-2 At Prometaphase
Specifically Localizes To The Axenome Of Motile Cilia As Opposed To Primary Non-Motile Cilia
Localization Is Independent Of Nubp2 And Kifc1
Alternative Antibody Names Anti-Cytosolic Fe-S Cluster Assembly Factor Nubp1 antibody
Anti-Nucleotide-Binding Protein 1 antibody
Anti-Nbp 1 antibody
Anti-NUBP1 antibody
Anti-NBP antibody
Anti-NBP1 antibody

Information sourced from Uniprot.org

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