• Western blot analysis of lysates from SH-SY5Y cells, using NRAP Rabbit polyclonal antibody (STJ114679) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-NRAP antibody (1-300) (STJ114679)

SKU:
STJ114679

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NRAP (1-300) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NRAP
Gene ID: 4892
Uniprot ID: NRAP_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NRAP (NP_001248392.1).
Immunogen Sequence: MNVQPCSRCGYGVYPAEKIS CIDQIWHKACFHCEVCKMML SVNNFVSHQKKPYCHAHNPK NNTFTSVYHTPLNLNVRTFP EAISGIHDQEDGEQCKSVFH WDMKSKDKEGAPNRQPLANE RAYWTGYGEGNAWCPGALPD PEIVRMVEARKSLGEEYTED YEQPRGKGSFPAMITPAYQR AKKANQLASQVEYKRGHDER ISRFSTVVDTPELLRSKAGA QLQSDVRYTEDYEQQRGKG
Tissue Specificity Expressed in cardiac and skeletal muscle.
Function May be involved in anchoring the terminal actin filaments in the myofibril to the membrane and in transmitting tension from the myofibrils to the extracellular matrix.
Protein Name Nebulin-Related-Anchoring Protein
N-Rap
Cellular Localisation Localized At The Myotendinous Junction In Skeletal Muscle And At The Intercalated Disk In Cardiac Muscle
Alternative Antibody Names Anti-Nebulin-Related-Anchoring Protein antibody
Anti-N-Rap antibody
Anti-NRAP antibody

Information sourced from Uniprot.org

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