Anti-NPM1 antibody (100-200 aa) [ABT358] (STJ197179)

SKU:
STJ197179

Current Stock:
Host: Mouse
Applications: IHC/WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Nucleophosmin (100-200 aa) is suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: ABT358
Conjugation: Unconjugated
Isotype: IgG2bk
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: IHC-P 1:100-500
WB 1:200-1000
IF 1:100-500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NPM1
Gene ID: 4869
Uniprot ID: NPM_HUMAN
Immunogen Region: 100-200 aa
Specificity: The antibody can specifically recognize human Nucleophosmin protein.
Immunogen: Synthesized peptide derived from the human Nucleophosmin at the amino acid range 100-200
Function Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade. In complex with MYC enhances the transcription of MYC target genes. May act as chaperonin or cotransporter in the nucleolar localization of transcription termination factor TTF1.
Protein Name Nucleophosmin
Npm
Nucleolar Phosphoprotein B23
Nucleolar Protein No38
Numatrin
Database Links Reactome: R-HSA-180746
Reactome: R-HSA-3899300
Reactome: R-HSA-606279
Reactome: R-HSA-6804115
Reactome: R-HSA-8869496
Reactome: R-HSA-9692914
Reactome: R-HSA-9700645
Reactome: R-HSA-9725370
Reactome: R-HSA-9725371
Cellular Localisation Nucleus
Nucleolus
Nucleoplasm
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Generally Nucleolar
But Is Translocated To The Nucleoplasm In Case Of Serum Starvation Or Treatment With Anticancer Drugs
Has Been Found In The Cytoplasm In Patients With Primary Acute Myelogenous Leukemia (Aml)
But Not With Secondary Aml
Can Shuttle Between Cytoplasm And Nucleus
Co- Localizes With The Methylated Form Of Rps10 In The Granular Component (Gc) Region Of The Nucleolus
Colocalized With Nucleolin And Apex1 In Nucleoli
Isoform 1 Of Nek2 Is Required For Its Localization To The Centrosome During Mitosis
Alternative Antibody Names Anti-Nucleophosmin antibody
Anti-Npm antibody
Anti-Nucleolar Phosphoprotein B23 antibody
Anti-Nucleolar Protein No38 antibody
Anti-Numatrin antibody
Anti-NPM1 antibody
Anti-NPM antibody

Information sourced from Uniprot.org

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