• Immunohistochemistry analysis of paraffin-embedded mouse kidney using NME2/NM23B antibody (STJ29579) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human kidney using NME2/NM23B antibody (STJ29579) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using NME2/NM23B antibody (STJ29579) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NME2 antibody (1-152) (STJ29579)

SKU:
STJ29579

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NME2
Gene ID: 4831/654364
Uniprot ID: NDKB_HUMAN
Immunogen Region: 1-152
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human NME2/NM23B (NP_001018148.1).
Immunogen Sequence: MANLERTFIAIKPDGVQRGL VGEIIKRFEQKGFRLVAMKF LRASEEHLKQHYIDLKDRPF FPGLVKYMNSGPVVAMVWEG LNVVKTGRVMLGETNPADSK PGTIRGDFCIQVGRNIIHGS DSVKSAEKEISLWFKPEELV DYKSCAHDWVYE
Tissue Specificity Isoform 1: Ubiquitously expressed. Isoform 3: Ubiquitously expressed.
Function Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene.binds DNA non-specifically. Binds to both single-stranded guanine- and cytosine-rich strands within the nuclease hypersensitive element (NHE) III(1) region of the MYC gene promoter. Does not bind to duplex NHE III(1). Has G-quadruplex (G4) DNA-binding activity, which is independent of its nucleotide-binding and kinase activity. Binds both folded and unfolded G4 with similar low nanomolar affinities. Stabilizes folded G4s regardless of whether they are prefolded or not. Exhibits histidine protein kinase activity.
Protein Name Nucleoside Diphosphate Kinase B
Ndk B
Ndp Kinase B
C-Myc Purine-Binding Transcription Factor Puf
Histidine Protein Kinase Ndkb
Nm23-H2
Database Links Reactome: R-HSA-499943
Reactome: R-HSA-6798695
Reactome: R-HSA-9748787
Reactome: R-HSA-9755088
Cellular Localisation Cytoplasm
Cell Projection
Lamellipodium
Ruffle
Colocalizes With Itgb1 And Itgb1bp1 At The Edge Or Peripheral Ruffles And Lamellipodia During The Early Stages Of Cell Spreading On Fibronectin Or Collagen But Not On Vitronectin Or Laminin Substrates
Isoform 1: Cytoplasm
Perinuclear Region
Nucleus
Isoform 3: Cytoplasm
Alternative Antibody Names Anti-Nucleoside Diphosphate Kinase B antibody
Anti-Ndk B antibody
Anti-Ndp Kinase B antibody
Anti-C-Myc Purine-Binding Transcription Factor Puf antibody
Anti-Histidine Protein Kinase Ndkb antibody
Anti-Nm23-H2 antibody
Anti-NME2 antibody
Anti-NM23B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance