• Confocal imaging of paraffin-embedded RAW 264. 7 cells using NINJ1 Rabbit monoclonal antibody (STJ11105438, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.

Anti-NINJ1 antibody (1-100) [S5438RM] (STJ11105438)

SKU:
STJ11105438

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Ninjurin-1 (1-100) is suitable for use in Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5438RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NINJ1
Gene ID: 4814
Uniprot ID: NINJ1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NINJ1 (NP_004139.2).
Immunogen Sequence: MDSGTEEYELNGGLPPGTPG SPDASPARWGWRHGPINVNH YASKKSAAESMLDIALLMAN ASQLKAVVEQGPSFAFYVPL VVLISISLVLQIGVGVLLIF
Tissue Specificity Widely expressed in both adult and embryonic tissues, primarily those of epithelial origin.
Post Translational Modifications Ninjurin-1: Cleaved by MMP9 protease to generate the Secreted ninjurin-1 form. Ninjurin-1: N-linked glycosylation is required for homooligomerization.
Function Ninjurin-1: Effector of necroptotic and pyroptotic programmed cell death that mediates plasma membrane rupture (cytolysis). Acts downstream of Gasdermin (GSDMA, GSDMB, GSDMC, GSDMD, or GSDME) or MLKL during pyroptosis or necroptosis, respectively: oligomerizes in response to death stimuli and promotes plasma membrane rupture by introducing hydrophilic faces of 2 alpha helices into the hydrophobic membrane, leading to release intracellular molecules named damage-associated molecular patterns (DAMPs) that propagate the inflammatory response. Acts as a regulator of Toll-like receptor 4 (TLR4) signaling triggered by lipopolysaccharide (LPS) during systemic inflammation.directly binds LPS. Involved in leukocyte migration during inflammation by promoting transendothelial migration of macrophages via homotypic binding. Promotes the migration of monocytes across the brain endothelium to central nervous system inflammatory lesions. Also acts as a homophilic transmembrane adhesion molecule involved in various processes such as axonal growth, cell chemotaxis and angiogenesis. Promotes cell adhesion by mediating homophilic interactions via its extracellular N-terminal adhesion motif (N-NAM). Involved in the progression of the inflammatory stress by promoting cell-to-cell interactions between immune cells and endothelial cells. Plays a role in nerve regeneration by promoting maturation of Schwann cells. Acts as a regulator of angiogenesis. Promotes the formation of new vessels by mediating the interaction between capillary pericyte cells and endothelial cells. Promotes osteoclasts development by enhancing the survival of prefusion osteoclasts. Also involved in striated muscle growth and differentiation. Secreted ninjurin-1: Secreted form generated by cleavage, which has chemotactic activity. Acts as an anti-inflammatory mediator by promoting monocyte recruitment, thereby ameliorating atherosclerosis.
Protein Name Ninjurin-1
Hninj1
Nerve Injury-Induced Protein 1 Cleaved Into - Secreted Ninjurin-1
Soluble Ninjurin-1
Cellular Localisation Ninjurin-1: Cell Membrane
Multi-Pass Membrane Protein
Synaptic Cell Membrane
Secreted Ninjurin-1: Secreted
Alternative Antibody Names Anti-Ninjurin-1 antibody
Anti-Hninj1 antibody
Anti-Nerve Injury-Induced Protein 1 Cleaved Into - Secreted Ninjurin-1 antibody
Anti-Soluble Ninjurin-1 antibody
Anti-NINJ1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance