• Immunoprecipitation analysis of 200 Mu g extracts of A-549 cells using 3 Mu g [KD Validated] NF2 Rabbit polyclonal antibody (STJ115585). Western blot was performed from the immunoprecipitate using [KO Validated] NF2 Rabbit polyclonal antibody (STJ115585) at a dilition of 1:1000.
  • Western blot analysis of lysates from wild type (WT) and NF2 knockdown (KD) HeLa cells, using [KD Validated] NF2 Rabbit polyclonal antibody (STJ115585) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of lysates from HepG2 cells, using [KD Validated] NF2 Rabbit polyclonal antibody (STJ115585) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-NF2 antibody (477-576) (STJ115585)

SKU:
STJ115585

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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NF2 (477-576) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NF2
Gene ID: 4771
Uniprot ID: MERL_HUMAN
Immunogen Region: 477-576
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 477-576 of human NF2 (NP_000259.1).
Immunogen Sequence: TKPTYPPMNPIPAPLPPDIP SFNLIGDSLSFDFKDTDMKR LSMEIEKEKVEYMEKSKHLQ EQLNELKTEIEALKLKERET ALDILHNENSDRGGSSKHNT
Tissue Specificity Widely expressed. Isoform 1 and isoform 3 are predominant. Isoform 4, isoform 5 and isoform 6 are expressed moderately. Isoform 8 is found at low frequency. Isoform 7, isoform 9 and isoform 10 are not expressed in adult tissues, with the exception of adult retina expressing isoform 10. Isoform 9 is faintly expressed in fetal brain, heart, lung, skeletal muscle and spleen. Fetal thymus expresses isoforms 1, 7, 9 and 10 at similar levels.
Post Translational Modifications Phosphorylation of Ser-518 inhibits nuclear localization by disrupting the intramolecular association of the FERM domain with the C-terminal tail. The dephosphorylation of Ser-518 favors the interaction with NOP53. Ubiquitinated by the CUL4A-RBX1-DDB1-DCAF1/VprBP E3 ubiquitin-protein ligase complex for ubiquitination and subsequent proteasome-dependent degradation.
Function Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.
Protein Name Merlin
Moesin-Ezrin-Radixin-Like Protein
Neurofibromin-2
Schwannomerlin
Schwannomin
Database Links Reactome: R-HSA-2029482
Reactome: R-HSA-5627123
Cellular Localisation Isoform 1: Cell Projection
Filopodium Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cell Projection
Ruffle Membrane
Nucleus
In A Fibroblastic Cell Line
Isoform 1 Is Found Homogeneously Distributed Over The Entire Cell
With A Particularly Strong Staining In Ruffling Membranes And Filopodia
Colocalizes With Mpp1 In Non-Myelin-Forming Schwann Cells
Binds With Dcaf1 In The Nucleus
The Intramolecular Association Of The Ferm Domain With The C-Terminal Tail Promotes Nuclear Accumulation
The Unphosphorylated Form Accumulates Predominantly In The Nucleus While The Phosphorylated Form Is Largely Confined To The Non-Nuclear Fractions
Isoform 7: Cytoplasm
Perinuclear Region
Cytoplasmic Granule
Observed In Cytoplasmic Granules Concentrated In A Perinuclear Location
Isoform 7 Is Absent From Ruffling Membranes And Filopodia
Isoform 9: Cytoplasm
Isoform 9 Is Absent From Ruffling Membranes And Filopodia
Isoform 10: Nucleus
Cytoplasm
Cytoskeleton
Isoform 10 Is Found Homogeneously Distributed Over The Entire Cell
Alternative Antibody Names Anti-Merlin antibody
Anti-Moesin-Ezrin-Radixin-Like Protein antibody
Anti-Neurofibromin-2 antibody
Anti-Schwannomerlin antibody
Anti-Schwannomin antibody
Anti-NF2 antibody
Anti-SCH antibody

Information sourced from Uniprot.org

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