| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NEU3 (222-461) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NEU3 |
| Gene ID: |
10825 |
| Uniprot ID: |
NEUR3_HUMAN |
| Immunogen Region: |
222-461 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 222-461 of human NEU3 (NP_006647.3). |
| Immunogen Sequence: |
CFQLPCKTRPHSLMIYSDDL GVTWHHGRLIRPMVTVECEV AEVTGRAGHPVLYCSARTPN RCRAEALSTDHGEGFQRLAL SRQLCEPPHGCQGSVVSFRP LEIPHRCQDSSSKDAPTIQQ SSPGSSLRLEEEAGTPSESW LLYSHPTSRKQRVDLGIYLN QTPLEAACWSRPWILHCGPC GYSDLAALEEEGLFGCLFEC GTKQECEQIAFRLFTHREIL SHLQGDCTSPGRNPSQFKS |
| Tissue Specificity | Highly expressed in skeletal muscle, testis, adrenal gland and thymus, followed by pancreas, liver, heart and thymus. Weakly expressed in kidney, placenta, brain and lung. |
| Post Translational Modifications | Palmitoylated.may regulate intracellular trafficking and anchorage to plasma membrane and endomembranes. |
| Function | Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides. Displays high catalytic efficiency for gangliosides including alpha-(2->3)-sialylated GD1a and GM3 and alpha-(2->8)-sialylated GD3. Plays a role in the regulation of transmembrane signaling through the modulation of ganglioside content of the lipid bilayer and by direct interaction with signaling receptors, such as EGFR. Desialylates EGFR and activates downstream signaling in proliferating cells. Contributes to clathrin-mediated endocytosis by regulating sorting of endocytosed receptors to early and recycling endosomes. |
| Protein Name | Sialidase-3Ganglioside SialidasedisMembrane SialidaseN-Acetyl-Alpha-Neuraminidase 3 |
| Database Links | Reactome: R-HSA-1660662Reactome: R-HSA-4085001 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinMembraneCaveolaEarly Endosome MembraneRecycling Endosome MembraneLysosome MembraneAssociates With The External Leaflet Of The Plasma MembraneS-Acylated Neu3 Likely Spans The Lipid Bilayer With A Portion Of C-Terminus Exposed To The Cytosol And The Catalytic Region Facing The Extracellular Space |
| Alternative Antibody Names | Anti-Sialidase-3 antibodyAnti-Ganglioside Sialidasedis antibodyAnti-Membrane Sialidase antibodyAnti-N-Acetyl-Alpha-Neuraminidase 3 antibodyAnti-NEU3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
