• Immunohistochemistry analysis of NEK2 in paraffin-embedded rat ovary using NEK2 Rabbit polyclonal antibody (STJ115297) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from HeLa cells, using NEK2 Rabbit polyclonal antibody (STJ115297) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NEK2 antibody (345-445) (STJ115297)

SKU:
STJ115297

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NEK2 (345-445) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NEK2
Gene ID: 4751
Uniprot ID: NEK2_HUMAN
Immunogen Region: 345-445
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 345-445 of human NEK2 (NP_002488.1).
Immunogen Sequence: LARAENLLKNYSLLKERKFL SLASNPELLNLPSSVIKKKV HFSGESKENIMRSENSESQL TSKSKCKDLKKRLHAAQLRA QALSDIEKNYQLKSRQILGM R
Tissue Specificity Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types. Isoform 1 and isoform 4 are expressed in the testis. Up-regulated in various cancer cell lines, as well as primary breast tumors.
Post Translational Modifications Activated by autophosphorylation. Protein phosphatase 1 represses autophosphorylation and activation of isoform 1 by dephosphorylation. Phosphorylation by STK3/MST2 is necessary for its localization to the centrosome.
Function Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC.SGO1.NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly. NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis. Involved in the regulation of centrosome disjunction. Phosphorylates CCDC102B either directly or indirectly which causes CCDC102B to dissociate from the centrosome and allows for centrosome separation. Isoform 1: Phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2: Not present in the nucleolus and, in contrast to isoform 1, does not phosphorylate and activate NEK11 in G1/S-arrested cells.
Protein Name Serine/Threonine-Protein Kinase Nek2
Hspk 21
Never In Mitosis A-Related Kinase 2
Nima-Related Protein Kinase 2
Nima-Like Protein Kinase 1
Database Links Reactome: R-HSA-179409
Reactome: R-HSA-2565942
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5620912
Reactome: R-HSA-8854518
Cellular Localisation Isoform 1: Nucleus
Nucleus
Nucleolus
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle Pole
Chromosome
Centromere
Kinetochore
Stk3/Mst2 And Sav1 Are Required For Its Targeting To The Centrosome
Colocalizes With Sgo1 And Mad1l1 At The Kinetochore
Not Associated With Kinetochore In The Interphase But Becomes Associated With It Upon The Breakdown Of The Nuclear Envelope
Has A Nucleolar Targeting/ Retention Activity Via A Coiled-Coil Domain At The C-Terminal End
Isoform 2: Cytoplasm
Predominantly Cytoplasmic
Isoform 4: Nucleus
Predominantly Nuclear
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Nek2 antibody
Anti-Hspk 21 antibody
Anti-Never In Mitosis A-Related Kinase 2 antibody
Anti-Nima-Related Protein Kinase 2 antibody
Anti-Nima-Like Protein Kinase 1 antibody
Anti-NEK2 antibody
Anti-NEK2A antibody
Anti-NLK1 antibody

Information sourced from Uniprot.org

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