• Western blot analysis of extracts of various cell lines, using NEDD8 antibody (STJ115481) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
  • Western blot analysis of extracts of various cell lines, using NEDD8 antibody (STJ115481) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
  • Anti-NEDD8 antibody (1-81) (STJ115481)

Anti-NEDD8 antibody (1-81) (STJ115481)

SKU:
STJ115481

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NEDD8 (1-81) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NEDD8
Gene ID: 4738
Uniprot ID: NEDD8_HUMAN
Immunogen Region: 1-81
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-81 of human NEDD8 (NP_006147.1).
Immunogen Sequence: MLIKVKTLTGKEIEIDIEPT DKVERIKERVEEKEGIPPQQ QRLIYSGKQMNDEKTAADYK ILGGSVLHLVLALRGGGGLR Q
Tissue Specificity Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
Post Translational Modifications Cleavage of precursor form by UCHL3 or SENP8 is necessary for function. (Microbial infection) Deamidated at Gln-40 by bacterial cyclomodulin Cif produced by enteropathogenic E.coli, Y.pseudotuberculosis or B.pseudomallei, leading to impair NEDD8 ability to activate cullin-RING-based E3 ubiquitin-protein ligase complexes (CRL complexes). Deamidation occurs on NEDD8-modified cullins. NEDD8 deamidation prevents switching from the inactive to active state by maintaining the 'closed' structure of the CRL complexes. Deamidation may also impair its deconjugation by the COP9 signalosome.However this result needs additional evidences.
Function Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53. Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Attachment of NEDD8 to p53/TP53 inhibits p53/TP53 transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M.
Protein Name Nedd8
Neddylin
Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8
Nedd-8
Ubiquitin-Like Protein Nedd8
Database Links Reactome: R-HSA-2173789
Reactome: R-HSA-5689603
Reactome: R-HSA-8856825
Reactome: R-HSA-8951664
Reactome: R-HSA-917937
Cellular Localisation Nucleus
Mainly Nuclear
Alternative Antibody Names Anti-Nedd8 antibody
Anti-Neddylin antibody
Anti-Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8 antibody
Anti-Nedd-8 antibody
Anti-Ubiquitin-Like Protein Nedd8 antibody
Anti-NEDD8 antibody

Information sourced from Uniprot.org

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