| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NDUFB5 (100-189) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NDUFB5 |
| Gene ID: |
4711 |
| Uniprot ID: |
NDUB5_HUMAN |
| Immunogen Region: |
100-189 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 100-189 of human NDUFB5 (NP_002483.1). |
| Immunogen Sequence: |
EIPEGYVPEHWEYYKHPISR WIARNFYDSPEKIYERTMAV LQIEAEKAELRVKELEVRKL MHVRGDGPWYYYETIDKELI DHSPKATPDN |
| Function | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. |
| Protein Name | Nadh Dehydrogenase Ubiquinone 1 Beta Subcomplex Subunit 5 - MitochondrialComplex I-SgdhCi-SgdhNadh-Ubiquinone Oxidoreductase Sgdh Subunit |
| Database Links | Reactome: R-HSA-611105Reactome: R-HSA-6799198 |
| Cellular Localisation | Mitochondrion Inner MembraneSingle-Pass Membrane ProteinMatrix Side |
| Alternative Antibody Names | Anti-Nadh Dehydrogenase Ubiquinone 1 Beta Subcomplex Subunit 5 - Mitochondrial antibodyAnti-Complex I-Sgdh antibodyAnti-Ci-Sgdh antibodyAnti-Nadh-Ubiquinone Oxidoreductase Sgdh Subunit antibodyAnti-NDUFB5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and NDUFB5 knockout (KO) HeLa cells, using [KO Validated] NDUFB5 Rabbit polyclonal antibody (STJ11100829) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89765/358239/STJ11100829_1__70027.1713122572.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NDUFB5 knockout (KO) HeLa cells, using [KO Validated] NDUFB5 Rabbit polyclonal antibody (STJ11100829) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
