• Western blot analysis of lysates from wild type (WT) and NDUFA13/GRIM19 knockout (KO) HeLa cells, using [KO Validated] NDUFA13/GRIM19 Rabbit polyclonal antibody (STJ11100044) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NDUFA13 antibody (52-144) (STJ11100044)

SKU:
STJ11100044

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDUFA13 (52-144) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFA13
Gene ID: 51079
Uniprot ID: NDUAD_HUMAN
Immunogen Region: 52-144
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 52-144 of human NDUFA13/GRIM19 (NP_057049.5).
Immunogen Sequence: KWNRERRRLQIEDFEARIAL LPLLQAETDRRTLQMLRENL EEEAIIMKDVPDWKVGESVF HTTRWVPPLIGELYGLRTTE EALHASHGFMWYT
Tissue Specificity Widely expressed, with highest expression in heart, skeletal muscle, liver, kidney and placenta. In intestinal mucosa, down-regulated in areas involved in Crohn disease and ulcerative colitis.
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1. Prevents the transactivation of STAT3 target genes. May play a role in CARD15-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes.
Protein Name Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Subunit 13
Cell Death Regulatory Protein Grim-19
Complex I-B16.6
Ci-B16.6
Gene Associated With Retinoic And Interferon-Induced Mortality 19 Protein
Grim-19
Gene Associated With Retinoic And Ifn-Induced Mortality 19 Protein
Nadh-Ubiquinone Oxidoreductase B16.6 Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Matrix Side
Nucleus
Localizes Mainly In The Mitochondrion
May Be Translocated Into The Nucleus Upon Ifn/Ra Treatment
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Subunit 13 antibody
Anti-Cell Death Regulatory Protein Grim-19 antibody
Anti-Complex I-B16.6 antibody
Anti-Ci-B16.6 antibody
Anti-Gene Associated With Retinoic And Interferon-Induced Mortality 19 Protein antibody
Anti-Grim-19 antibody
Anti-Gene Associated With Retinoic And Ifn-Induced Mortality 19 Protein antibody
Anti-Nadh-Ubiquinone Oxidoreductase B16.6 Subunit antibody
Anti-NDUFA13 antibody
Anti-GRIM19 antibody
Anti-CDA016 antibody
Anti-CGI-39 antibody

Information sourced from Uniprot.org

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