• Western blot analysis of extracts of various cell lines, using NCK1 antibody (STJ115295) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-NCK1 antibody (58-377) (STJ115295)

SKU:
STJ115295

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NCK1 (58-377) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NCK1
Gene ID: 4690
Uniprot ID: NCK1_HUMAN
Immunogen Region: 58-377
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 58-377 of human NCK1 (NP_006144.1).
Immunogen Sequence: RKNSARKASIVKNLKDTLGI GKVKRKPSVPDSASPADDSF VDPGERLYDLNMPAYVKFNY MAEREDELSLIKGTKVIVME KCSDGWWRGSYNGQVGWFPS NYVTEEGDSPLGDHVGSLSE KLAAVVNNLNTGQVLHVVQA LYPFSSSNDEELNFEKGDVM DVIEKPENDPEWWKCRKING MVGLVPKNYVTVMQNNPLTS GLEPSPPQCDYIRPSLTGKF AGNPWYYGKVTRHQAEMAL
Post Translational Modifications Phosphorylated on Ser and Tyr residues. Phosphorylated in response to activation of EGFR and FcERI. Phosphorylated by activated PDGFRB.
Function Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors.
Protein Name Cytoplasmic Protein Nck1
Nck Adaptor Protein 1
Nck-1
Sh2/Sh3 Adaptor Protein Nck-Alpha
Database Links Reactome: R-HSA-186763
Reactome: R-HSA-202433
Reactome: R-HSA-2029482
Reactome: R-HSA-373753
Reactome: R-HSA-418885
Reactome: R-HSA-428540
Reactome: R-HSA-4420097
Reactome: R-HSA-5663213
Reactome: R-HSA-9013420
Reactome: R-HSA-9013424
Reactome: R-HSA-9664422
Reactome: R-HSA-9679191
Reactome: R-HSA-983695
Cellular Localisation Cytoplasm
Endoplasmic Reticulum
Nucleus
Mostly Cytoplasmic
But Shuttles Between The Cytoplasm And The Nucleus
Import Into The Nucleus Requires The Interaction With Socs7
Predominantly Nuclear Following Genotoxic Stresses
Such As Uv Irradiation
Hydroxyurea Or Mitomycin C Treatments
Alternative Antibody Names Anti-Cytoplasmic Protein Nck1 antibody
Anti-Nck Adaptor Protein 1 antibody
Anti-Nck-1 antibody
Anti-Sh2/Sh3 Adaptor Protein Nck-Alpha antibody
Anti-NCK1 antibody
Anti-NCK antibody

Information sourced from Uniprot.org

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