• Immunohistochemistry analysis of NAT8 in paraffin-embedded human stomach using NAT8 Rabbit polyclonal antibody (STJ110070) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of NAT8 in paraffin-embedded rat kidney using NAT8 Rabbit polyclonal antibody (STJ110070) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of NAT8 in paraffin-embedded mouse brain using NAT8 Rabbit polyclonal antibody (STJ110070) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using NAT8 Rabbit polyclonal antibody (STJ110070) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NAT8 antibody (61-220) (STJ110070)

SKU:
STJ110070

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NAT8 (61-220) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NAT8
Gene ID: 9027
Uniprot ID: NAT8_HUMAN
Immunogen Region: 61-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 61-220 of human NAT8 (Q9UHE5).
Immunogen Sequence: LLALVFSISLFPALWFLAKK PWTEYVDMTLCTDMSDITKS YLSERGSCFWVAESEEKVVG MVGALPVDDPTLREKRLQLF HLFVDSEHRRQGIAKALVRT VLQFARDQGYSEVILDTGTI QLSAMALYQSMGFKKTGQSF FCVWARLVALHTVHFIYHLP
Tissue Specificity Preferentially expressed in liver and kidney. Also detected in brain (at protein level).
Function Acetylates the free alpha-amino group of cysteine S-conjugates to form mercapturic acids. This is the final step in a major route for detoxification of a wide variety of reactive electrophiles which starts with their incorporation into glutathione S-conjugates. The glutathione S-conjugates are then further processed into cysteine S-conjugates and finally mercapturic acids which are water soluble and can be readily excreted in urine or bile. Alternatively, may have a lysine N-acetyltransferase activity catalyzing peptidyl-lysine N6-acetylation of various proteins. Thereby, may regulate apoptosis through the acetylation and the regulation of the expression of PROM1. May also regulate amyloid beta-peptide secretion through acetylation of BACE1 and the regulation of its expression in neurons.
Protein Name N-Acetyltransferase 8
Acetyltransferase 2
Atase2
Camello-Like Protein 1
Cysteinyl-Conjugate N-Acetyltransferase
Ccnat
Database Links Reactome: R-HSA-977225
Cellular Localisation Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane
Single-Pass Type Ii Membrane Protein
Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-N-Acetyltransferase 8 antibody
Anti-Acetyltransferase 2 antibody
Anti-Atase2 antibody
Anti-Camello-Like Protein 1 antibody
Anti-Cysteinyl-Conjugate N-Acetyltransferase antibody
Anti-Ccnat antibody
Anti-NAT8 antibody
Anti-CML1 antibody
Anti-GLA antibody
Anti-TSC501 antibody

Information sourced from Uniprot.org

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