• Western blot analysis of extracts of normal 293T cells and 293T transfected with Myc-NLK protein, using Myc-Tag antibody (STJ11103700) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunoprecipitation of NLK-Myc from 300 µg extracts of 293F cells transfected with a NLK expression vector containing a single C-terminal Myc-Tag was performed using 3 µg of Mouse anti Myc-Tag monoclonal antibody (STJ11103700). Mouse IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10 % of the total input. Western blot analysis of immunoprecipitates was conducted using Mouse anti Myc-Tag monoclonal antibody at a dilution of 1:3000.
  • Immunofluorescence analysis of 293T cells transfected with Myc-Tag fusion protein and untreated 293T cells use mouse anti Myc-Tag monoclonal antibody (STJ11103700) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of normal 293T cells and 293T transfected with Myc-NLK protein, using Mouse anti Myc-Tag monoclonal antibody (STJ11103700) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug Myc-Tag antibody (STJ11103700). Western blot was performed from the immunoprecipitate using Myc-Tag (STJ11103700) at a dilition of 1:1000.

Anti-Myc-Tag antibody [S0MM] (STJ11103700)

SKU:
STJ11103700

£135.50 - £249.50
Current Stock:
Host: Mouse
Applications: WB/IF/IP/ELISA
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S0MM
Conjugation: Unconjugated
Isotype: IgG1
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:2000-1:6000
IF:1:1000-1:5000
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Specificity: A synthetic peptide corresponding to Myc tag.
Immunogen Sequence: EQKLISEEDL
Background Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance