• Western blot analysis of extracts of various cell lines, using MTO1 antibody (STJ111580) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MTO1 antibody (420-680) (STJ111580)

SKU:
STJ111580

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MTO1 (420-680) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MTO1
Gene ID: 25821
Uniprot ID: MTO1_HUMAN
Immunogen Region: 420-680
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 420-680 of human MTO1 (NP_036255.2).
Immunogen Sequence: QGVIAGINASLRVSRKPPFV VSRTEGYIGVLIDDLTTLGT SEPYRMFTSRVEFRLSLRPD NADSRLTLRGYKDAGCVSQQ RYERACWMKSSLEEGISVLK SIEFLSSKWKKLIPEASIST SRSLPVRALDVLKYEEVDMD SLAKAVPEPLKKYTKCRELA ERLKIEATYESVLFHQLQEI KGVQQDEALQLPKDLDYLTI RDVSLSHEVREKLHFSRPQT IGAASRIPGVTPAAIINLL
Tissue Specificity Ubiquitously expressed in various tissues, but with a markedly elevated expression in tissues of high metabolic rates including cochlea.
Function Involved in the 5-carboxymethylaminomethyl modification (mnm(5)s(2)U34) of the wobble uridine base in mitochondrial tRNAs.
Protein Name Protein Mto1 Homolog - Mitochondrial
Database Links Reactome: R-HSA-6787450
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Protein Mto1 Homolog - Mitochondrial antibody
Anti-MTO1 antibody
Anti-CGI-02 antibody

Information sourced from Uniprot.org

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