• Immunofluorescence analysis of L929 cells using MT-ATP6 Rabbit polyclonal antibody (STJ119939) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using MT-ATP6 Rabbit polyclonal antibody (STJ119939) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MT-ATP6 antibody (27-41) (STJ119939)

SKU:
STJ119939

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MT-ATP6 (27-41) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MT-ATP6
Gene ID: 4508
Uniprot ID: ATP6_HUMAN
Immunogen Region: 27-41
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 27-41 of human MT-ATP6 (YP_003024031.1).
Immunogen Sequence: PPLLIPTSKYLINNR
Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Key component of the proton channel.it may play a direct role in the translocation of protons across the membrane.
Protein Name Atp Synthase Subunit A
F-Atpase Protein 6
Database Links Reactome: R-HSA-163210
Reactome: R-HSA-8949613
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Atp Synthase Subunit A antibody
Anti-F-Atpase Protein 6 antibody
Anti-MT-ATP6 antibody
Anti-ATP6 antibody
Anti-ATPASE6 antibody
Anti-MTATP6 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance