• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using MRPL13 antibody (STJ116057) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using MRPL13 antibody (STJ116057) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MRPL13 antibody (1-178) (STJ116057)

SKU:
STJ116057

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MRPL13 (1-178) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MRPL13
Gene ID: 28998
Uniprot ID: RM13_HUMAN
Immunogen Region: 1-178
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-178 of human MRPL13 (NP_054797.2).
Immunogen Sequence: MSSFSRAPQQWATFARIWYL LDGKMQPPGKLAAMASIRLQ GLHKPVYHALSDCGDHVVIM NTRHIAFSGNKWEQKVYSSH TGYPGGFRQVTAAQLHLRDP VAIVKLAIYGMLPKNLHRRT MMERLHLFPDEYIPEDILKN LVEELPQPRKIPKRLDEYTQ EEIDAFPRLWTPPEDYRL
Protein Name Large Ribosomal Subunit Protein Ul13m
39s Ribosomal Protein L13 - Mitochondrial
L13mt
Mrp-L13
Database Links Reactome: R-HSA-5368286
Reactome: R-HSA-5389840
Reactome: R-HSA-5419276
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein Ul13m antibody
Anti-39s Ribosomal Protein L13 - Mitochondrial antibody
Anti-L13mt antibody
Anti-Mrp-L13 antibody
Anti-MRPL13 antibody

Information sourced from Uniprot.org

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