| Host: | Rabbit |
| Applications: | WB/ELISA/IHC |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-MMS19 nucleotide excision repair protein homolog (653-703 aa) is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 IHC-P 1:50-300 ELISA 2000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MMS19 |
| Gene ID: | 64210 |
| Uniprot ID: | MMS19_HUMAN |
| Immunogen Region: | 653-703 aa |
| Specificity: | This antibody detects endogenous levels of MMS19 at Human/Mouse |
| Immunogen: | Synthesized peptide derived from the human MMS19 at the amino acid range 653-703 |
| Post Translational Modifications | Ubiquitinated.undergoes 'Lys-48'-linked polyubiquitination by MAGEF1-NSMCE1 ubiquitin ligase complex leading to proteasomal degradation. |
| Function | Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity. In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins such as ERCC2/XPD, FANCJ and RTEL1, thereby playing a key role in nucleotide excision repair (NER), homologous recombination-mediated double-strand break DNA repair, DNA replication and RNA polymerase II (POL II) transcription. As part of the mitotic spindle-associated MMXD complex, plays a role in chromosome segregation, probably by facilitating iron-sulfur (Fe-S) cluster assembly into ERCC2/XPD. Together with CIAO2, facilitates the transfer of Fe-S clusters to the motor protein KIF4A, which ensures proper localization of KIF4A to mitotic machinery components to promote the progression of mitosis. Indirectly acts as a transcriptional coactivator of estrogen receptor (ER), via its role in iron-sulfur insertion into some component of the TFIIH-machinery. |
| Protein Name | Mms19 Nucleotide Excision Repair Protein Homolog Hmms19 Met18 Homolog Mms19-Like Protein |
| Database Links | Reactome: R-HSA-2564830 |
| Cellular Localisation | Nucleus Cytoplasm Cytoskeleton Spindle Microtubule Organizing Center Centrosome In Mitosis Enriched On Centrosomes During Prophase Localizes To The Spindle During Metaphase And Surrounds Compacted Spindle Midzone Microtubules During Telophase |
| Alternative Antibody Names | Anti-Mms19 Nucleotide Excision Repair Protein Homolog antibody Anti-Hmms19 antibody Anti-Met18 Homolog antibody Anti-Mms19-Like Protein antibody Anti-MMS19 antibody Anti-MMS19L antibody |
Information sourced from Uniprot.org