• Confocal imaging of PC-12 cells using MMP3 Rabbit monoclonal antibody (STJ11101327, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo®488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Confocal imaging of U-87 MG cells using MMP3 Rabbit monoclonal antibody (STJ11101327, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo®488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Confocal imaging of A549 cells using MMP3 Rabbit monoclonal antibody (STJ11101327, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry analysis of MMP3 in paraffin-embedded human liver tissue using MMP3 Rabbit monoclonal antibody (STJ11101327) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MMP3 in paraffin-embedded human liver cancer tissue using MMP3 Rabbit monoclonal antibody (STJ11101327) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from Mouse lung, using MMP3 Rabbit monoclonal antibody (STJ11101327) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Western blot analysis of lysates from U-87MG cells, using MMP3 Rabbit monoclonal antibody (STJ11101327) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-MMP3 antibody (385-477) [S7MR] (STJ11101327)

SKU:
STJ11101327

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-MMP3 (385-477) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MMP3
Gene ID: 4314
Uniprot ID: MMP3_HUMAN
Immunogen Region: 385-477
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 385-477 of human MMP3 (NP_002413.1).
Immunogen Sequence: VRKIDAAISDKEKNKTYFFV EDKYWRFDEKRNSMEPGFPK QIAEDFPGIDSKIDAVFEEF GFFYFFTGSSQLEFDPNAKK VTHTLKSNSWLNC
Post Translational Modifications Directly cleaved by HTRA2 to produce active form.
Function Metalloproteinase with a rather broad substrate specificity that can degrade fibronectin, laminin, gelatins of type I, III, IV, and V.collagens III, IV, X, and IX, and cartilage proteoglycans. Activates different molecules including growth factors, plasminogen or other matrix metalloproteinases such as MMP9. Once released into the extracellular matrix (ECM), the inactive pro-enzyme is activated by the plasmin cascade signaling pathway. Acts also intracellularly. For example, in dopaminergic neurons, gets activated by the serine protease HTRA2 upon stress and plays a pivotal role in DA neuronal degeneration by mediating microglial activation and alpha-synuclein/SNCA cleavage. In addition, plays a role in immune response and possesses antiviral activity against various viruses such as vesicular stomatitis virus, influenza A virus (H1N1) and human herpes virus 1. Mechanistically, translocates from the cytoplasm into the cell nucleus upon virus infection to influence NF-kappa-B activities.
Protein Name Stromelysin-1
Sl-1
Matrix Metalloproteinase-3
Mmp-3
Transin-1
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Reactome: R-HSA-1592389
Reactome: R-HSA-2022090
Reactome: R-HSA-2179392
Reactome: R-HSA-6785807
Reactome: R-HSA-9009391
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Nucleus
Cytoplasm
Alternative Antibody Names Anti-Stromelysin-1 antibody
Anti-Sl-1 antibody
Anti-Matrix Metalloproteinase-3 antibody
Anti-Mmp-3 antibody
Anti-Transin-1 antibody
Anti-MMP3 antibody
Anti-STMY1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance