• Western blot analysis of extracts of SKOV3 cells, using MMP1 antibody (STJ24572) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MMP1 antibody (20-469) (STJ24572)

SKU:
STJ24572

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MMP1 (20-469) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MMP1
Gene ID: 4312
Uniprot ID: MMP1_HUMAN
Immunogen Region: 20-469
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-469 of human MMP1 (NP_002412.1).
Immunogen Sequence: FPATLETQEQDVDLVQKYLE KYYNLKNDGRQVEKRRNSGP VVEKLKQMQEFFGLKVTGKP DAETLKVMKQPRCGVPDVAQ FVLTEGNPRWEQTHLTYRIE NYTPDLPRADVDHAIEKAFQ LWSNVTPLTFTKVSEGQADI MISFVRGDHRDNSPFDGPGG NLAHAFQPGPGIGGDAHFDE DERWTNNFREYNLHRVAAHE LGHSLGLSHSTDIGALMYPS YTFSGDVQLAQDDIDGIQA
Post Translational Modifications Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase. Tyrosine phosphorylated in platelets by PKDCC/VLK.
Function Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
Protein Name Interstitial Collagenase
Fibroblast Collagenase
Matrix Metalloproteinase-1
Mmp-1 Cleaved Into - 22 Kda Interstitial Collagenase - 27 Kda Interstitial Collagenase
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Reactome: R-HSA-1592389
Reactome: R-HSA-210991
Reactome: R-HSA-381426
Reactome: R-HSA-6785807
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Interstitial Collagenase antibody
Anti-Fibroblast Collagenase antibody
Anti-Matrix Metalloproteinase-1 antibody
Anti-Mmp-1 Cleaved Into - 22 Kda Interstitial Collagenase - 27 Kda Interstitial Collagenase antibody
Anti-MMP1 antibody
Anti-CLG antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance