• Immunofluorescence analysis of HeLa cells using Ki67 Rabbit polyclonal antibody (STJ119231) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of A-431 cells using Ki67 Rabbit polyclonal antibody (STJ119231) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using Ki67 Rabbit polyclonal antibody (STJ119231) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Ki67 in paraffin-embedded human colon carcinoma tissue using Ki67 Rabbit polyclonal antibody (STJ119231) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Ki67 in paraffin-embedded human tonsil tissue using Ki67 Rabbit polyclonal antibody (STJ119231) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using Ki67 Rabbit polyclonal antibody (STJ119231) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180.

Anti-MKI67 antibody (460-680) (STJ119231)

SKU:
STJ119231

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Ki67 (460-680) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MKI67
Gene ID: 4288
Uniprot ID: KI67_HUMAN
Immunogen Region: 460-680
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 460-680 of human Ki67 (NP_002408.3).
Immunogen Sequence: SLSKPEKLGTTAGQMCSGLP GLSSVDINNFGDSINESEGI PLKRRRVSFGGHLRPELFDE NLPPNTPLKRGEAPTKRKSL VMHTPPVLKKIIKEQPQPSG KQESGSEIHVEVKAQSLVIS PPAPSPRKTPVASDQRRRSC KTAPASSSKSQTEVPKRGGR KSGNLPSKRVSISRSQHDIL QMICSKRRSGASEANLIVAK SWADVVKLGAKQTQTKVIKH G
Post Translational Modifications Phosphorylated. Hyperphosphorylated in mitosis. Hyperphosphorylated form does not bind DNA.
Function Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization. It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Protein Name Proliferation Marker Protein Ki-67
Antigen Identified By Monoclonal Antibody Ki-67
Antigen Ki-67
Antigen Ki67
Cellular Localisation Chromosome
Nucleus
Nucleolus
Associates With The Surface Of The Mitotic Chromosome
The Perichromosomal Layer
And Covers A Substantial Fraction Of The Mitotic Chromosome Surface
Associates With Satellite Dna In G1 Phase
Binds Tightly To Chromatin In Interphase
Chromatin-Binding Decreases In Mitosis When It Associates With The Surface Of The Condensed Chromosomes
Predominantly Localized In The G1 Phase In The Perinucleolar Region
In The Later Phases It Is Also Detected Throughout The Nuclear Interior
Being Predominantly Localized In The Nuclear Matrix
Alternative Antibody Names Anti-Proliferation Marker Protein Ki-67 antibody
Anti-Antigen Identified By Monoclonal Antibody Ki-67 antibody
Anti-Antigen Ki-67 antibody
Anti-Antigen Ki67 antibody
Anti-MKI67 antibody

Information sourced from Uniprot.org

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