• Immunofluorescence analysis of U-87MG cells using [KD Validated] MIF Rabbit polyclonal antibody (STJ11104919) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts from wild type (WT) and MIF knockdown (KD) 293T (KD) cells, using MIF antibody (STJ11104919) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates, using MIF antibody (STJ11104919) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MIF antibody (STJ11104919)

SKU:
STJ11104919

£129.50 - £639.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Macrophage Migration Inhibitory Factor is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MIF
Gene ID: 4282
Uniprot ID: MIF_HUMAN
Immunogen: Recombinant protein of human MIF.
Function Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
Protein Name Macrophage Migration Inhibitory Factor
Mif
Glycosylation-Inhibiting Factor
Gif
L-Dopachrome Isomerase
L-Dopachrome Tautomerase
Phenylpyruvate Tautomerase
Database Links Reactome: R-HSA-202733
Reactome: R-HSA-6798695
Reactome: R-HSA-8950505
Cellular Localisation Secreted
Cytoplasm
Does Not Have A Cleavable Signal Sequence And Is Secreted Via A Specialized
Non-Classical Pathway
Secreted By Macrophages Upon Stimulation By Bacterial Lipopolysaccharide (Lps)
Or By M
Tuberculosis Antigens
Alternative Antibody Names Anti-Macrophage Migration Inhibitory Factor antibody
Anti-Mif antibody
Anti-Glycosylation-Inhibiting Factor antibody
Anti-Gif antibody
Anti-L-Dopachrome Isomerase antibody
Anti-L-Dopachrome Tautomerase antibody
Anti-Phenylpyruvate Tautomerase antibody
Anti-MIF antibody
Anti-GLIF antibody
Anti-MMIF antibody

Information sourced from Uniprot.org

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