• Western blot analysis of extracts of A-549 cells, using MGAT4B antibody (STJ114676) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MGAT4B antibody (409-548) (STJ114676)

SKU:
STJ114676

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MGAT4B (409-548) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MGAT4B
Gene ID: 11282
Uniprot ID: MGT4B_HUMAN
Immunogen Region: 409-548
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 409-548 of human MGAT4B (NP_055090.1).
Immunogen Sequence: TYQHFTLEKAYLREDFFWAF TPAAGDFIRFRFFQPLRLER FFFRSGNIEHPEDKLFNTSV EVLPFDNPQSDKEALQEGRT ATLRYPRSPDGYLQIGSFYK GVAEGEVDPAFGPLEALRLS IQTDSPVWVILSEIFLKKAD
Tissue Specificity Widely expressed. Strongly overexpressed in pancreatic cancer.
Post Translational Modifications N-glycosylated.
Function Glycosyltransferase that catalyzes the transfer of GlcNAc from UDP-GlcNAc to the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans through a beta1-4 linkage and participates in the production of tri- and tetra-antennary N-linked sugar chains. Prefers complex-type N-glycans over hybrid-types. Has lower affinities for donors or acceptors than MGAT4A, suggesting that, under physiological conditions, it is not the main contributor in N-glycan biosynthesis.
Protein Name Alpha-1 -3-Mannosyl-Glycoprotein 4-Beta-N-Acetylglucosaminyltransferase B
N-Glycosyl-Oligosaccharide-Glycoprotein N-Acetylglucosaminyltransferase Ivb
Glcnac-T Ivb
Gnt-Ivb
N-Acetylglucosaminyltransferase Ivb
Udp-N-Acetylglucosamine - Alpha-1 -3-D-Mannoside Beta-1 -4-N-Acetylglucosaminyltransferase Ivb
Database Links Reactome: R-HSA-9694548
Reactome: R-HSA-975577
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type Ii Membrane Protein
A Processed Soluble Form Also Exists
Alternative Antibody Names Anti-Alpha-1 -3-Mannosyl-Glycoprotein 4-Beta-N-Acetylglucosaminyltransferase B antibody
Anti-N-Glycosyl-Oligosaccharide-Glycoprotein N-Acetylglucosaminyltransferase Ivb antibody
Anti-Glcnac-T Ivb antibody
Anti-Gnt-Ivb antibody
Anti-N-Acetylglucosaminyltransferase Ivb antibody
Anti-Udp-N-Acetylglucosamine - Alpha-1 -3-D-Mannoside Beta-1 -4-N-Acetylglucosaminyltransferase Ivb antibody
Anti-MGAT4B antibody
Anti-UNQ906 antibody
Anti-PRO1927 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance