• Immunofluorescence analysis of L929 cells using MGAT1 Rabbit polyclonal antibody (STJ28470) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using MGAT1 Rabbit polyclonal antibody (STJ28470) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MGAT1 antibody (156-445) (STJ28470)

SKU:
STJ28470

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MGAT1 (156-445) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MGAT1
Gene ID: 4245
Uniprot ID: MGAT1_HUMAN
Immunogen Region: 156-445
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 156-445 of human MGAT1 (NP_001108092.1).
Immunogen Sequence: GSAVTHIRQPDLSSIAVPPD HRKFQGYYKIARHYRWALGQ VFRQFRFPAAVVVEDDLEVA PDFFEYFRATYPLLKADPSL WCVSAWNDNGKEQMVDASRP ELLYRTDFFPGLGWLLLAEL WAELEPKWPKAFWDDWMRRP EQRQGRACIRPEISRTMTFG RKGVSHGQFFDQHLKFIKLN QQFVHFTQLDLSYLQREAYD RDFLARVYGAPQLQVEKVRT NDRKELGEVRVQYTGRDSF
Function Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.
Protein Name Alpha-1 -3-Mannosyl-Glycoprotein 2-Beta-N-Acetylglucosaminyltransferase
N-Glycosyl-Oligosaccharide-Glycoprotein N-Acetylglucosaminyltransferase I
Gnt-I
Glcnac-T I
Database Links Reactome: R-HSA-964739
Reactome: R-HSA-9683686
Reactome: R-HSA-9694548
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type Ii Membrane Protein
Cytoplasm
Perinuclear Region
Co-Localizes With Bri3 Isoform 1 At The Perinuclear Region
Alternative Antibody Names Anti-Alpha-1 -3-Mannosyl-Glycoprotein 2-Beta-N-Acetylglucosaminyltransferase antibody
Anti-N-Glycosyl-Oligosaccharide-Glycoprotein N-Acetylglucosaminyltransferase I antibody
Anti-Gnt-I antibody
Anti-Glcnac-T I antibody
Anti-MGAT1 antibody
Anti-GGNT1 antibody
Anti-GLCT1 antibody
Anti-GLYT1 antibody
Anti-MGAT antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance