• Confocal imaging of paraffin-embedded Mouse brain tissue using Mitofusin 2 Rabbit monoclonal antibody (STJ11101760, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of various lysates using Mitofusin 2 Rabbit monoclonal antibody (STJ11101760) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MFN2 antibody (1-100) [S0MR] (STJ11101760)

SKU:
STJ11101760

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Mitofusin 2 (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MFN2
Gene ID: 9927
Uniprot ID: MFN2_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Mitofusin 2 (O95140).
Immunogen Sequence: MSLLFSRCNSIVTVKKNKRH MAEVNASPLKHFVTAKKKIN GIFEQLGAYIQESATFLEDT YRNAELDPVTTEEQVLDVKG YLSKVRGISEVLARRHMKVA
Tissue Specificity Ubiquitous.expressed at low level. Highly expressed in heart and kidney.
Post Translational Modifications Phosphorylated by PINK1. Ubiquitinated by non-degradative ubiquitin by PRKN, promoting mitochondrial fusion.deubiquitination by USP30 inhibits mitochondrial fusion. Ubiquitinated by HUWE1 when dietary stearate (C18:0) levels are low.ubiquitination inhibits mitochondrial fusion.
Function Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion. Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks. Membrane clustering requires GTPase activity and may involve a major rearrangement of the coiled coil domains (Probable). Plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PRKN recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions.
Protein Name Mitofusin-2
Transmembrane Gtpase Mfn2
Database Links Reactome: R-HSA-5205685
Reactome: R-HSA-9013419
Reactome: R-HSA-983231
Cellular Localisation Mitochondrion Outer Membrane
Multi-Pass Membrane Protein
Colocalizes With Bax During Apoptosis
Alternative Antibody Names Anti-Mitofusin-2 antibody
Anti-Transmembrane Gtpase Mfn2 antibody
Anti-MFN2 antibody
Anti-CPRP1 antibody
Anti-KIAA0214 antibody

Information sourced from Uniprot.org

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