• Western blot analysis of various lysates, using MFAP5 antibody (STJ11104913) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MFAP5 antibody (73-173 aa) (STJ11104913)

SKU:
STJ11104913

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MFAP5
Gene ID: 8076
Uniprot ID: MFAP5_HUMAN
Immunogen Region: 73-173 aa
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 73-173 of human MFAP5. (NP_003471.1).
Immunogen Sequence: ASLSEKNTTAECWDEKFTCT RLYSVHRPVKQCIHQLCFTS LRRMYIVNKEICSRLVCKEH EAMKDELCRQMAGLPPRRLR RSNYFRLPPCENVDLQRPNG L
Post Translational Modifications Forms intermolecular disulfide bonds either with other MAGP-2 molecules or with other components of the microfibrils. N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. O-glycan heterogeneity at Thr-54: HexHexNAc (major) and HexHexNAc + sulfate (minor).
Function May play a role in hematopoiesis. In the cardiovascular system, could regulate growth factors or participate in cell signaling in maintaining large vessel integrity. Component of the elastin-associated microfibrils.
Protein Name Microfibrillar-Associated Protein 5
Mfap-5
Mp25
Microfibril-Associated Glycoprotein 2
Magp-2
Database Links Reactome: R-HSA-1566948
Reactome: R-HSA-2129379
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Microfibrillar-Associated Protein 5 antibody
Anti-Mfap-5 antibody
Anti-Mp25 antibody
Anti-Microfibril-Associated Glycoprotein 2 antibody
Anti-Magp-2 antibody
Anti-MFAP5 antibody
Anti-MAGP2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance