| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-MCM4 (100-200) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MCM4 |
| Gene ID: |
4173 |
| Uniprot ID: |
MCM4_HUMAN |
| Immunogen Region: |
100-200 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human MCM4 (P33991). |
| Immunogen Sequence: |
EGTPRSGVRGTPVRQRPDLG SAQKGLQVDLQSDGAAAEDI VASEQSLGQKLVIWGTDVNV AACKENFQRFLQRFIDPLAK EEENVGIDITEPLYMQRLGE I |
| Post Translational Modifications | Sumoylated.SUMO2 modified in response to stress caused by inhibition of proteasome activity (in vitro). |
| Function | Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. |
| Protein Name | Dna Replication Licensing Factor Mcm4Cdc21 HomologP1-Cdc21 |
| Database Links | Reactome: R-HSA-176187Reactome: R-HSA-176974Reactome: R-HSA-68867Reactome: R-HSA-68949Reactome: R-HSA-68962Reactome: R-HSA-69052 |
| Cellular Localisation | NucleusChromosomeAssociated With Chromatin Before The Formation Of Nuclei And Detaches From It As Dna Replication Progresses |
| Alternative Antibody Names | Anti-Dna Replication Licensing Factor Mcm4 antibodyAnti-Cdc21 Homolog antibodyAnti-P1-Cdc21 antibodyAnti-MCM4 antibodyAnti-CDC21 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
