• Western blot analysis of lysates from mouse spleen, using MCM3AP Rabbit polyclonal antibody (STJ118193) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-MCM3AP antibody (250-550) (STJ118193)

SKU:
STJ118193

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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MCM3AP (250-550) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MCM3AP
Gene ID: 8888
Uniprot ID: GANP_HUMAN
Immunogen Region: 250-550
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 250-550 of human MCM3AP (NP_003897.2).
Immunogen Sequence: SSFPVSSAVLGEPFQASKAG VRQGCEEAVSQVEPLPSLMK GLKRKEDQDRSPRRHGHEPA EDSDPLSRGDHPPDKRPVRL NRPRGGTLFGRTIQDVFKSN KEVGRLGNKEAKKETGFVES AESDHMAIPGGNQSVLAPSR IPGVNKEEETESREKKEDSL RGTPARQSNRSESTDSLGGL SPSEVTAIQCKNIPDYLNDR TILENHFGKIAKVQRIFTRR SKKLAVVHFFDHASAALAR
Tissue Specificity Widely expressed. Up-regulated in germinal center B-cells in tonsils (at protein level).
Function Isoform GANP: As a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores. Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations. Isoform MCM3AP: Binds to and acetylates the replication protein MCM3. Plays a role in the initiation of DNA replication and participates in controls that ensure that DNA replication initiates only once per cell cycle. Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations.
Protein Name Germinal-Center Associated Nuclear Protein
Ganp
80 Kda Mcm3-Associated Protein
Mcm3 Acetylating Protein
Mcm3ap
Mcm3 Acetyltransferase
Cellular Localisation Isoform Ganp: Nucleus Envelope
Nucleus
Nuclear Pore Complex
Nucleoplasm
Chromosome
Predominantly Located At The Nuclear Envelope
Facing The Nucleus Interior
Localization At The Nuclear Pore Complex Requires Nup153
Tpr And Alyref/Aly
Also Found Associated With Chromatin
In B-Cells
Targeted To The Immunoglobulin Variable Region Genes
Isoform Mcm3ap: Cytoplasm
Translocates Into The Nucleus In The Presence Of Mcm3
Associates With Chromatin Possibly Through Interaction With Mcm3
Alternative Antibody Names Anti-Germinal-Center Associated Nuclear Protein antibody
Anti-Ganp antibody
Anti-80 Kda Mcm3-Associated Protein antibody
Anti-Mcm3 Acetylating Protein antibody
Anti-Mcm3ap antibody
Anti-Mcm3 Acetyltransferase antibody
Anti-MCM3AP antibody
Anti-GANP antibody
Anti-KIAA0572 antibody
Anti-MAP80 antibody

Information sourced from Uniprot.org

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