| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-MASTL (580-879) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MASTL |
| Gene ID: |
84930 |
| Uniprot ID: |
GWL_HUMAN |
| Immunogen Region: |
580-879 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 580-879 of human MASTL (NP_001165774.1). |
| Immunogen Sequence: |
ESPLESQPLDSDRSIKESSF EESNIEDPLIVTPDCQEKTS PKGVENPAVQESNQKMLGPP LEVLKTLASKRNAVAFRSFN SHINASNNSEPSRMNMTSLD AMDISCAYSGSYPMAITPTQ KRRSCMPHQQTPNQIKSGTP YRTPKSVRRGVAPVDDGRIL GTPDYLAPELLLGRAHGPAV DWWALGVCLFEFLTGIPPFN DETPQQVFQNILKRDIPWPE GEEKLSDNAQSAVEILLTI |
| Post Translational Modifications | Phosphorylation at Thr-741 by CDK1 during M phase activates its kinase activity. Maximum phosphorylation occurs in prometaphase. |
| Function | Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro.however such activity is unsure in vivo. May be involved in megakaryocyte differentiation. |
| Protein Name | Serine/Threonine-Protein Kinase GreatwallGwGwlHgwlMicrotubule-Associated Serine/Threonine-Protein Kinase-LikeMast-L |
| Database Links | Reactome: R-HSA-2465910 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeNucleusCleavage FurrowDuring Interphase Is Mainly NuclearUpon Nuclear Envelope Breakdown Localizes At The Cytoplasm And During Mitosis At The CentrosomesUpon Mitotic Exit Moves To The Cleavage Furrow |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Greatwall antibodyAnti-Gw antibodyAnti-Gwl antibodyAnti-Hgwl antibodyAnti-Microtubule-Associated Serine/Threonine-Protein Kinase-Like antibodyAnti-Mast-L antibodyAnti-MASTL antibodyAnti-GW antibodyAnti-GWL antibodyAnti-THC2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
