• Immunohistochemistry analysis of Mast Cell Tryptase (TPSB2) in paraffin-embedded human colon using Mast Cell Tryptase (TPSB2) Rabbit monoclonal antibody (STJ11103144) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Mast Cell Tryptase (TPSB2) Rabbit monoclonal antibody (STJ11103144) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Mast Cell Tryptase antibody (30-275) [S4MR] (STJ11103144)

SKU:
STJ11103144

£146.50 - £642.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:2000
IHC-P:1:100-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TPSAB1
Gene ID: 7177
Uniprot ID: TRYB1_HUMAN
Immunogen Region: 30-275
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 30-275 of human Mast Cell Tryptase (TPSB2) (TPSB2) (P20231).
Immunogen Sequence: GIVGGQEAPRSKWPWQVSLR VHGPYWMHFCGGSLIHPQWV LTAAHCVGPDVKDLAALRVQ LREQHLYYQDQLLPVSRIIV HPQFYTAQIGADIALLELEE PVNVSSHVHTVTLPPASETF PPGMPCWVTGWGDVDNDERL PPPFPLKQVKVPIMENHICD AKYHLGAYTGDDVRIVRDDM LCAGNTRRDSCQGDSGGPLV CKVNGTWLQAGVVSWGEGCA QPNRPGIYTRVTYYLDWIH
Tissue Specificity Isoform 1 and isoform 2 are expressed in lung, stomach, spleen, heart and skin.in these tissues, isoform 1 is predominant. Isoform 2 is expressed in aorta, spleen, and breast tumor, with highest levels in the endothelial cells of some blood vessels surrounding the aorta, as well as those surrounding the tumor and low levels, if any, in mast cells (at protein level).
Function Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates.
Protein Name Tryptase Alpha/Beta-1
Tryptase-1
Tryptase I
Tryptase Alpha-1
Database Links Reactome: R-HSA-1592389
Cellular Localisation Secreted
Released From The Secretory Granules Upon Mast Cell Activation
Alternative Antibody Names Anti-Tryptase Alpha/Beta-1 antibody
Anti-Tryptase-1 antibody
Anti-Tryptase I antibody
Anti-Tryptase Alpha-1 antibody
Anti-TPSAB1 antibody
Anti-TPS1 antibody
Anti-TPS2 antibody
Anti-TPSB1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance