| Host: |
Rabbit |
| Applications: |
IHC/IF/ELISA |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Mitogen-activated protein kinase 15 (361-410 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
IHC 1:100-1:300IF 1:200-1:1000ELISA 1:5000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MAPK15 |
| Gene ID: |
225689 |
| Uniprot ID: |
MK15_HUMAN |
| Immunogen Region: |
361-410 aa |
| Specificity: |
ERK 8 Polyclonal Antibody detects endogenous levels of ERK 8 protein. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human MAPK15 at the amino acid range 361-410 |
| Post Translational Modifications | Autophosphorylated on Thr-175 and Tyr-177.activates the enzyme. Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase. |
| Function | Atypical MAPK protein that regulates several process such as autophagy, ciliogenesis, protein trafficking/secretion and genome integrity, in a kinase activity-dependent manner. Controls both, basal and starvation-induced autophagy throught its interaction with GABARAP, MAP1LC3B and GABARAPL1 leading to autophagosome formation, SQSTM1 degradation and reduced MAP1LC3B inhibitory phosphorylation. Regulates primary cilium formation and the localization of ciliary proteins involved in cilium structure, transport, and signaling. Prevents the relocation of the sugar-adding enzymes from the Golgi to the endoplasmic reticulum, thereby restricting the production of sugar-coated proteins. Upon amino-acid starvation, mediates transitional endoplasmic reticulum site disassembly and inhibition of secretion. Binds to chromatin leading to MAPK15 activation and interaction with PCNA, that which protects genomic integrity by inhibiting MDM2-mediated degradation of PCNA. Regulates DA transporter (DAT) activity and protein expression via activation of RhoA. In response to H(2)O(2) treatment phosphorylates ELAVL1, thus preventing it from binding to the PDCD4 3'UTR and rendering the PDCD4 mRNA accessible to miR-21 and leading to its degradation and loss of protein expression. Also functions in a kinase activity-independent manner as a negative regulator of growth. Phosphorylates in vitro FOS and MBP. During oocyte maturation, plays a key role in the microtubule organization and meiotic cell cycle progression in oocytes, fertilized eggs, and early embryos. Interacts with ESRRA promoting its re-localization from the nucleus to the cytoplasm and then prevents its transcriptional activity. |
| Protein Name | Mitogen-Activated Protein Kinase 15Map Kinase 15Mapk 15Extracellular Signal-Regulated Kinase 7Erk-7Extracellular Signal-Regulated Kinase 8Erk-8 |
| Cellular Localisation | CytoplasmCytoskeletonCilium Basal BodyCell JunctionTight JunctionMicrotubule Organizing CenterCentrosomeCentrioleCytoplasmic VesicleAutophagosomeGolgi ApparatusNucleusSpindleCo-Localizes To The Cytoplasm Only In Presence Of EsrraTranslocates To The Nucleus Upon ActivationAt Prometaphase IMetaphase I (Mi)Anaphase ITelophase IAnd Metaphase Ii (Mii) StagesIs Stably Detected At The Spindle |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase 15 antibodyAnti-Map Kinase 15 antibodyAnti-Mapk 15 antibodyAnti-Extracellular Signal-Regulated Kinase 7 antibodyAnti-Erk-7 antibodyAnti-Extracellular Signal-Regulated Kinase 8 antibodyAnti-Erk-8 antibodyAnti-MAPK15 antibodyAnti-ERK7 antibodyAnti-ERK8 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
