• Immunofluorescence analysis of L-929 cells using MAP3K1 Rabbit polyclonal antibody (STJ11104371) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of MAP3K1 in paraffin-embedded rat liver tissue using MAP3K1 Rabbit polyclonal antibody (STJ11104371) at a dilution of 1:100 (40x lens). Microwave antigen retrieval was performed with 0. 01 M Tris-EDTA repair solution (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MAP3K1 in paraffin-embedded rat spleen tissue using MAP3K1 Rabbit polyclonal antibody (STJ11104371) at a dilution of 1:100 (40x lens). Microwave antigen retrieval was performed with 0. 01 M Tris-EDTA repair solution (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MAP3K1 in paraffin-embedded mouse spleen tissue using MAP3K1 Rabbit polyclonal antibody (STJ11104371) at a dilution of 1:100 (40x lens). Microwave antigen retrieval was performed with 0. 01 M Tris-EDTA repair solution (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MAP3K1 in paraffin-embedded mouse liver tissue using MAP3K1 Rabbit polyclonal antibody (STJ11104371) at a dilution of 1:100 (40x lens). Microwave antigen retrieval was performed with 0. 01 M Tris-EDTA repair solution (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MAP3K1 in paraffin-embedded human esophagus tissue using MAP3K1 Rabbit polyclonal antibody (STJ11104371) at a dilution of 1:100 (40x lens). Microwave antigen retrieval was performed with 0. 01 M Tris-EDTA repair solution (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MAP3K1 in paraffin-embedded human liver cancer tissue using MAP3K1 Rabbit polyclonal antibody (STJ11104371) at a dilution of 1:100 (40x lens). Microwave antigen retrieval was performed with 0. 01 M Tris-EDTA repair solution (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MAP3K1 in paraffin-embedded human lung cancer tissue using MAP3K1 Rabbit polyclonal antibody (STJ11104371) at a dilution of 1:100 (40x lens). Microwave antigen retrieval was performed with 0. 01 M Tris-EDTA repair solution (pH 9. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using MAP3K1 (STJ11104371) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MAP3K1 antibody (1400-1512) (STJ11104371)

SKU:
STJ11104371

£116.50 - £543.50
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 (1400-1512) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP3K1
Gene ID: 4214
Uniprot ID: M3K1_HUMAN
Immunogen Region: 1400-1512
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1400-1512 of human MAP3K1 (NP_005912.1).
Immunogen Sequence: TGAGEFQGQLLGTIAFMAPE VLRGQQYGRSCDVWSVGCAI IEMACAKPPWNAEKHSNHLA LIFKIASATTAPSIPSHLSP GLRDVALRCLELQPQDRPPS RELLKHPVFRTTW
Post Translational Modifications Autophosphorylated.
Function Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. May phosphorylate the MAPK8/JNK1 kinase. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.
Protein Name Mitogen-Activated Protein Kinase Kinase Kinase 1
Mapk/Erk Kinase Kinase 1
Mek Kinase 1
Mekk 1
Database Links Reactome: R-HSA-166058
Reactome: R-HSA-2871796
Reactome: R-HSA-933542
Reactome: R-HSA-975138
Reactome: R-HSA-975871
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 antibody
Anti-Mapk/Erk Kinase Kinase 1 antibody
Anti-Mek Kinase 1 antibody
Anti-Mekk 1 antibody
Anti-MAP3K1 antibody
Anti-MAPKKK1 antibody
Anti-MEKK antibody
Anti-MEKK1 antibody

Information sourced from Uniprot.org

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