• Immunofluorescence analysis of PC-3 cells using MEK7 Rabbit polyclonal antibody (STJ11104998) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of Rat skeletal muscle, using MEK7 antibody (STJ11104998) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MAP2K7 antibody (STJ11104998)

SKU:
STJ11104998

Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 7 is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:20-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP2K7
Gene ID: 5609
Uniprot ID: MP2K7_HUMAN
Immunogen: Recombinant protein of human MEK7.
Immunogen Sequence: DPPDPTKPDYDIRADVWSLG ISLVELATGQFPYKNCKTDF EVLTKVLQEEPPLLPGHMGF SGDFQSFVKDCLTKDHRKRP KYNKLLEHSFIKRYETLEVD VASWFKDVMAKTESPRTSGV LSQPHLPFFR
Tissue Specificity Ubiquitous.with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle.
Post Translational Modifications Activated by phosphorylation on Ser-271 and Thr-275 by MAP kinase kinase kinases (MAP3Ks).
Function Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by pro-inflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Part of a non-canonical MAPK signaling pathway, composed of the upstream MAP3K12 kinase and downstream MAP kinases MAPK1/ERK2 and MAPK3/ERK1, that enhances the AP-1-mediated transcription of APP in response to APOE.
Protein Name Dual Specificity Mitogen-Activated Protein Kinase Kinase 7
Map Kinase Kinase 7
Mapkk 7
Jnk-Activating Kinase 2
Mapk/Erk Kinase 7
Mek 7
Stress-Activated Protein Kinase Kinase 4
Sapk Kinase 4
Sapkk-4
Sapkk4
C-Jun N-Terminal Kinase Kinase 2
Jnk Kinase 2
Jnkk 2
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-2871796
Reactome: R-HSA-450321
Reactome: R-HSA-5210891
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 7 antibody
Anti-Map Kinase Kinase 7 antibody
Anti-Mapkk 7 antibody
Anti-Jnk-Activating Kinase 2 antibody
Anti-Mapk/Erk Kinase 7 antibody
Anti-Mek 7 antibody
Anti-Stress-Activated Protein Kinase Kinase 4 antibody
Anti-Sapk Kinase 4 antibody
Anti-Sapkk-4 antibody
Anti-Sapkk4 antibody
Anti-C-Jun N-Terminal Kinase Kinase 2 antibody
Anti-Jnk Kinase 2 antibody
Anti-Jnkk 2 antibody
Anti-MAP2K7 antibody
Anti-JNKK2 antibody
Anti-MEK7 antibody
Anti-MKK7 antibody
Anti-PRKMK7 antibody
Anti-SKK4 antibody

Information sourced from Uniprot.org

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