• Immunoprecipitation analysis of 300 Mu g extracts of NIH/3T3 cells using 3 Mu g MAP1LC3A antibody (STJ11101405). Western blot was performed from the immunoprecipitate using MAP1LC3A antibody (STJ11101405) at a dilution of 1:1000. NIH/3T3 cells were treated by Chloroquine (50 Mu M) at 37 °C for 20 hours.
  • Western blot analysis of lysates from SH-SY5Y cells using MAP1LC3A Rabbit monoclonal antibody (STJ11101405) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:10s.
  • Western blot analysis of lysates from NIH/3T3, C6 cells using MAP1LC3A Rabbit monoclonal antibody (STJ11101405) at 1:1000 dilution. NIH/3T3 cells and C6 cells were treated by Chloroquine (50 Mu M) at 37 °C for 20 hours. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 180s.

Anti-MAP1LC3A antibody (1-100) [S5MR] (STJ11101405)

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STJ11101405

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Host: Rabbit
Applications: WB/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-MAP1LC3A (1-100) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IP 1:50-1:1200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP1LC3A
Gene ID: 84557
Uniprot ID: MLP3A_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP1LC3A (Q9H492).
Immunogen Sequence: MPSDRPFKQRRSFADRCKEV QQIRDQHPSKIPVIIERYKG EKQLPVLDKTKFLVPDHVNM SELVKIIRRRLQLNPTQAFF LLVNQHSMVSVSTPIADIYE
Tissue Specificity Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
Post Translational Modifications The precursor molecule is cleaved by ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) to expose the glycine at the C-terminus and form the cytosolic form, LC3-I. The processed form is then activated by APG7L/ATG7, transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) phospholipid to form the membrane-bound form, LC3-II. During non-canonical autophagy, the processed form is conjugated to phosphatidylserine (PS) phospholipid. ATG4 proteins also mediate the delipidation of PE-conjugated forms. In addition, ATG4B and ATG4D mediate delipidation of ATG8 proteins conjugated to PS during non-canonical autophagy. ATG4B constitutes the major protein for proteolytic activation. ATG4D is the main enzyme for delipidation activity. (Microbial infection) The Legionella effector RavZ is a deconjugating enzyme that hydrolyzes the amide bond between the C-terminal glycine residue and an adjacent aromatic residue in ATG8 proteins conjugated to phosphatidylethanolamine (PE), producing an ATG8 protein that is resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine. RavZ is also able to mediate delipidation of ATG8 proteins conjugated to phosphatidylserine (PS). Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE).
Function Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover.
Protein Name Microtubule-Associated Proteins 1a/1b Light Chain 3a
Autophagy-Related Protein Lc3 A
Autophagy-Related Ubiquitin-Like Modifier Lc3 A
Map1 Light Chain 3-Like Protein 1
Map1a/Map1b Light Chain 3 A
Map1a/Map1b Lc3 A
Microtubule-Associated Protein 1 Light Chain 3 Alpha
Database Links Reactome: R-HSA-1632852
Reactome: R-HSA-5205685
Reactome: R-HSA-8934903
Cellular Localisation Cytoplasmic Vesicle
Autophagosome Membrane
Lipid-Anchor
Endomembrane System
Cytoplasm
Cytoskeleton
Lc3-Ii Binds To The Autophagic Membranes
Alternative Antibody Names Anti-Microtubule-Associated Proteins 1a/1b Light Chain 3a antibody
Anti-Autophagy-Related Protein Lc3 A antibody
Anti-Autophagy-Related Ubiquitin-Like Modifier Lc3 A antibody
Anti-Map1 Light Chain 3-Like Protein 1 antibody
Anti-Map1a/Map1b Light Chain 3 A antibody
Anti-Map1a/Map1b Lc3 A antibody
Anti-Microtubule-Associated Protein 1 Light Chain 3 Alpha antibody
Anti-MAP1LC3A antibody

Information sourced from Uniprot.org

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