• Immunohistochemistry analysis of paraffin-embedded human colon using Monoamine Oxidase A (MAOA) Rabbit monoclonal antibody (STJ11101997) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Rat liver, using Monoamine Oxidase A (MAOA) Rabbit monoclonal antibody (STJ11101997) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using Monoamine Oxidase A (MAOA) Rabbit monoclonal antibody (STJ11101997) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MAOA antibody (428-527) [S7MR] (STJ11101997)

SKU:
STJ11101997

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Monoamine Oxidase A (428-527) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAOA
Gene ID: 4128
Uniprot ID: AOFA_HUMAN
Immunogen Region: 428-527
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 428-527 of human Monoamine Oxidase A (MAOA) (P21397).
Immunogen Sequence: GRIFFAGTETATKWSGYMEG AVEAGERAAREVLNGLGKVT EKDIWVQEPESKDVPAVEIT HTFWERNLPSVSGLLKIIGF STSVTALGFVLYKYKLLPRS
Tissue Specificity Heart, liver, duodenum, blood vessels and kidney.
Function Catalyzes the oxidative deamination of primary and some secondary amine such as neurotransmitters, with concomitant reduction of oxygen to hydrogen peroxide and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. Preferentially oxidizes serotonin. Also catalyzes the oxidative deamination of kynuramine to 3-(2-aminophenyl)-3-oxopropanal that can spontaneously condense to 4-hydroxyquinoline.
Protein Name Amine Oxidase Flavin-Containing A
Monoamine Oxidase Type A
Mao-A
Database Links Reactome: R-HSA-141333
Reactome: R-HSA-181430
Reactome: R-HSA-379397
Reactome: R-HSA-379398
Reactome: R-HSA-379401
Reactome: R-HSA-380612
Reactome: R-HSA-5579012
Reactome: R-HSA-6785807
Cellular Localisation Mitochondrion Outer Membrane
Single-Pass Type Iv Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Amine Oxidase Flavin-Containing A antibody
Anti-Monoamine Oxidase Type A antibody
Anti-Mao-A antibody
Anti-MAOA antibody

Information sourced from Uniprot.org

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