| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MAGOH |
| Gene ID: | 4116 |
| Uniprot ID: | MGN_HUMAN |
| Immunogen Region: | 1-146 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-146 of human MAGOH (NP_002361.1). |
| Immunogen Sequence: | MESDFYLRYYVGHKGKFGHE FLEFEFRPDGKLRYANNSNY KNDVMIRKEAYVHKSVMEEL KRIIDDSEITKEDDALWPPP DRVGRQELEIVIGDEHISFT TSKIGSLIDVNQSKDPEGLR VFYYLVQDLKCLVFSLIGLH FKIKPI |
| Tissue Specificity | Ubiquitous. |
| Function | Required for pre-mRNA splicing as component of the spliceosome. Plays a redundant role with MAGOHB as core component of the exon junction complex (EJC) and in the nonsense-mediated decay (NMD) pathway. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S pre-initiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes).specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S).the function is different from the established EJC assembly. |
| Protein Name | Protein Mago Nashi Homolog |
| Database Links | Reactome: R-HSA-159236 Reactome: R-HSA-72163 Reactome: R-HSA-72187 Reactome: R-HSA-73856 Reactome: R-HSA-9010553 Reactome: R-HSA-975957 |
| Cellular Localisation | Nucleus Nucleus Speckle Cytoplasm Detected In Granule-Like Structures In The Dendroplasm Travels To The Cytoplasm As Part Of The Exon Junction Complex (Ejc) Bound To Mrna Colocalizes With The Core Ejc Alyref/Thoc4 Nxf1 And Uap56 In The Nucleus And Nuclear Speckles |
| Alternative Antibody Names | Anti-Protein Mago Nashi Homolog antibody Anti-MAGOH antibody Anti-MAGOHA antibody |
Information sourced from Uniprot.org