• Western blot analysis of various lysates using MAF1 Rabbit polyclonal antibody (STJ117726) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MAF1 antibody (1-256) (STJ117726)

SKU:
STJ117726

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MAF1 (1-256) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAF1
Gene ID: 84232
Uniprot ID: MAF1_HUMAN
Immunogen Region: 1-256
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-256 of human MAF1 (NP_115648.2).
Immunogen Sequence: MKLLENSSFEAINSQLTVET GDAHIIGRIESYSCKMAGDD KHMFKQFCQEGQPHVLEALS PPQTSGLSPSRLSKSQGGEE EGPLSDKCSRKTLFYLIATL NESFRPDYDFSTARSHEFSR EPSLSWVVNAVNCSLFSAVR EDFKDLKPQLWNAVDEEICL AECDIYSYNPDLDSDPFGED GSLWSFNYFFYNKRLKRIVF FSCRSISGSTYTPSEAGNEL DMELGEEEVEEESRSGGSG
Post Translational Modifications Phosphorylated at Ser-60, Ser-68 and Ser-75.the major sites of phosphorylation. Nuclear accumulation correlates with a concomitant dephosphorylation. Phosphorylation may attenuate its RNA polymerase III-repressive function. Sumoylated with SUMO1 and SUMO2, mainly on Lys-35. Desumoylated by SENP1. SUMOylation promotes the ability of MAF1 to repress transcription and suppress colony formation.
Function Plays a role in the repression of RNA polymerase III-mediated transcription in response to changing nutritional, environmental and cellular stress conditions to balance the production of highly abundant tRNAs, 5S rRNA, and other small non-coding RNAs with cell growth and maintenance. Also plays a key role in cell fate determination by promoting mesorderm induction and adipocyte differentiation. Mechanistically, associates with the RNA polymerase III clamp and thereby impairs its recruitment to the complex made of the promoter DNA, TBP and the initiation factor TFIIIB. When nutrients are available and mTOR kinase is active, MAF1 is hyperphosphorylated and RNA polymerase III is engaged in transcription. Stress-induced MAF1 dephosphorylation results in nuclear localization, increased targeting of gene-bound RNA polymerase III and a decrease in the transcriptional readout. Additionally, may also regulate RNA polymerase I and RNA polymerase II-dependent transcription through its ability to regulate expression of the central initiation factor TBP.
Protein Name Repressor Of Rna Polymerase Iii Transcription Maf1 Homolog
Database Links Reactome: R-HSA-8943724
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Repressor Of Rna Polymerase Iii Transcription Maf1 Homolog antibody
Anti-MAF1 antibody

Information sourced from Uniprot.org

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