• Immunohistochemistry analysis of Lamin B1 in paraffin-embedded mouse brain using Lamin B1 Rabbit polyclonal antibody (STJ11105386) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Lamin B1 in paraffin-embedded human oophoroma using Lamin B1 Rabbit polyclonal antibody (STJ11105386) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Lamin B1 in paraffin-embedded rat brain using Lamin B1 Rabbit polyclonal antibody (STJ11105386) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from HeLa cells, using Lamin B1 Rabbit polyclonal antibody (STJ11105386). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-LMNB1 antibody (400-500) (STJ11105386)

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STJ11105386

£151.50 - £599.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Lamin-B1 (400-500) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LMNB1
Gene ID: 4001
Uniprot ID: LMNB1_HUMAN
Immunogen Region: 400-500
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 400-500 of human Lamin B1 (NP_005564.1).
Immunogen Sequence: VSRASSSRSVRTTRGKRKRV DVEESEASSSVSISHSASAT GNVCIEEIDVDGKFIRLKNT SEQDQPMGGWEMIRKIGDTS VSYKYTSRYVLKAGQTVTIW A
Post Translational Modifications B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
Function Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Protein Name Lamin-B1
Database Links Reactome: R-HSA-1221632
Reactome: R-HSA-2559584
Reactome: R-HSA-2980766
Reactome: R-HSA-2995383
Reactome: R-HSA-352238
Reactome: R-HSA-4419969
Reactome: R-HSA-8862803
Reactome: R-HSA-8950505
Reactome: R-HSA-9013405
Reactome: R-HSA-9035034
Cellular Localisation Nucleus Lamina
Alternative Antibody Names Anti-Lamin-B1 antibody
Anti-LMNB1 antibody
Anti-LMN2 antibody
Anti-LMNB antibody

Information sourced from Uniprot.org

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