| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-Lipocalin 2/NGAL-Mouse (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:8000. WB: Approx 23kDa band observed in Mouse Kidney lysates (calculated MW of 22.6kDa according to NP_005555.2). Recommended concentration: 1-3µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Immunogen Region: | Internal |
| Accession Number: | NP_032517.1 |
| Immunogen Sequence: | HRYPQVQSYNVQ |
| Post Translational Modifications | N-glycosylated. |
| Function | Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity.limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin. Can also bind siderophores from M.tuberculosis. |
| Protein Name | Neutrophil Gelatinase-Associated Lipocalin Ngal Lipocalin-2 Oncogene 24p3 24p3 Sv-40-Induced 24p3 Protein Siderocalin Lcn2 P25 |
| Database Links | Reactome: R-MMU-6798695 Reactome: -MMU-6799990 Reactome: -MMU-917937 |
| Cellular Localisation | Secreted Cytoplasmic Granule Lumen Cytoplasmic Vesicle Lumen Upon Binding To The Slc22a17 (24p3r) Receptor It Is Internalized Releases The Bound Iron In The Acidic Lumen Of Cytoplasmic Vesicles |
| Alternative Antibody Names | Anti-Neutrophil Gelatinase-Associated Lipocalin antibody Anti-Ngal antibody Anti-Lipocalin-2 antibody Anti-Oncogene 24p3 antibody Anti-24p3 antibody Anti-Sv-40-Induced 24p3 Protein antibody Anti-Siderocalin Lcn2 antibody Anti-P25 antibody Anti-Lcn2 antibody |
Information sourced from Uniprot.org