| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-LIN28B (161-250) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
LIN28B |
| Gene ID: |
389421 |
| Uniprot ID: |
LN28B_HUMAN |
| Immunogen Region: |
161-250 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 161-250 of human LIN28B (NP_001004317.1). |
| Immunogen Sequence: |
VANCPHKNVAQPPASSQGRQ EAESQPCTSTLPREVGGGHG CTSPPFPQEARAEISERSGR SPQEASSTKSSIAPEEQSKK GPSVQKRKKT |
| Tissue Specificity | Expressed at high levels in the placenta and, at mucher lower, in testis and fetal liver. Isoform 1 is only detected in placenta and in moderately and poorly differentiated hepatocellular carcinoma cells (at protein level). Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues (at protein level). Tends to be up-regulated in triple-negative (ER-,PR-,HER2-) breast tumors, as well as in liver, ovarian, and thyroid carcinomas. |
| Function | Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA. Does not act on pri-miR21. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation. This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent. Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression. When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. |
| Protein Name | Protein Lin-28 Homolog BLin-28b |
| Cellular Localisation | NucleusNucleolusCytoplasmPredominantly NucleolarIn Huh7 CellsPredominantly CytoplasmicWith Only A Subset Of Cells Exhibiting Strong Nuclear StainingHoweverThe Specificity Of The Polyclonal Antibody Used In These Experiments Has Not Been Not Documented |
| Alternative Antibody Names | Anti-Protein Lin-28 Homolog B antibodyAnti-Lin-28b antibodyAnti-LIN28B antibodyAnti-CSDD2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
