| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:5000 IHC-P:1:100-1:500 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | LGR5 |
| Gene ID: | 8549 |
| Uniprot ID: | LGR5_HUMAN |
| Immunogen Region: | 824-907 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 824-907 of human LGR5/GPR49 (NP_003658.1). |
| Immunogen Sequence: | NPHFKEDLVSLRKQTYVWTR SKHPSLMSINSDDVEKQSCD STQALVTFTSSSITYDLPPS SVPSPAYPVTESCHLSSVAF VPCL |
| Tissue Specificity | Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver. |
| Function | Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development. |
| Protein Name | Leucine-Rich Repeat-Containing G-Protein Coupled Receptor 5 G-Protein Coupled Receptor 49 G-Protein Coupled Receptor 67 G-Protein Coupled Receptor Hg38 |
| Database Links | Reactome: R-HSA-4641263 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Golgi Apparatus Trans-Golgi Network Membrane Rapidly And Constitutively Internalized To The Trans-Golgi Network At Steady State Internalization To The Trans-Golgi Network May Be The Result Of Phosphorylation At Ser-861 And Ser-864 However The Phosphorylation Event Has Not Been Proven |
| Alternative Antibody Names | Anti-Leucine-Rich Repeat-Containing G-Protein Coupled Receptor 5 antibody Anti-G-Protein Coupled Receptor 49 antibody Anti-G-Protein Coupled Receptor 67 antibody Anti-G-Protein Coupled Receptor Hg38 antibody Anti-LGR5 antibody Anti-GPR49 antibody Anti-GPR67 antibody |
Information sourced from Uniprot.org