| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-LEMD2 (200-300) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
LEMD2 |
| Gene ID: |
221496 |
| Uniprot ID: |
LEMD2_HUMAN |
| Immunogen Region: |
200-300 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human LEMD2 (NP_851853.1). |
| Immunogen Sequence: |
PEVGRRLERWLSRLLLWASL GLLLVFLGILWVKMGKPSAP QEAEDNMKLLPVDCERKTDE FCQAKQKAALLELLHELYNF LAIQAGNFECGNPENLKSKC I |
| Tissue Specificity | Ubiquitously expressed, including bone marrow, brain, kidney, colon, skeletal muscle, thymus, testis and uterus. |
| Post Translational Modifications | Phosphorylated.strongly phosphorylated in mitosis compared to G1/S. |
| Function | Nuclear lamina-associated inner nuclear membrane protein that is involved in nuclear structure organization, maintenance of nuclear envelope (NE) integrity and NE reformation after mitosis. Plays a role as transmembrane adapter for the endosomal sorting complexes required for transport (ESCRT), and is thereby involved in ESCRT-mediated NE reformation. Promotes ESCRT-mediated NE closure by recruiting CHMP7 and downstream ESCRT-III proteins IST1/CHMP8 and CHMP2A to the reforming NE during anaphase. During nuclear reassembly, condenses into a liquid-like coating around microtubule spindles and coassembles with CHMP7 to form a macromolecular O-ring seal at the confluence between membranes, chromatin, and the spindle to facilitate early nuclear sealing. Plays a role in the organization of heterochromatin associated with the NE and in the maintenance of NE organization under mechanical stress. Required for embryonic development and involved in regulation of several signaling pathways such as MAPK and AKT. Required for myoblast differentiation involving regulation of ERK signaling. Essential for cardiac homeostasis and proper heart function. |
| Protein Name | Lem Domain-Containing Protein 2Hlem2 |
| Database Links | Reactome: R-HSA-2980766Reactome: R-HSA-2995383Reactome: R-HSA-4419969Reactome: R-HSA-9668328 |
| Cellular Localisation | Nucleus Inner MembraneMulti-Pass Membrane ProteinNucleus EnvelopeCytoplasmCytoskeletonSpindleLamina-Associated Protein Residing In The Inner Nuclear Membrane (Inm) Of The Nuclear Envelope (Ne)The Localization To The Inm Is Dependent On LmnaEvenly Distributed Around The Ne During InterphaseDuring MetaphaseFound In A Reticular NetworkRecruited To The Reforming Ne On Chromatin Disks In Early AnaphaseIn Late AnaphaseConcentrates At The Ne Core Proximal To Spindle MicrotubulesAnd Then Broadening To A Distributed Nuclear Rim Pattern |
| Alternative Antibody Names | Anti-Lem Domain-Containing Protein 2 antibodyAnti-Hlem2 antibodyAnti-LEMD2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
