• Western blot analysis of extracts of various cell lines, using LEFTY2 antibody (STJ116000) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-LEFTY2 antibody (57-366) (STJ116000)

SKU:
STJ116000

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LEFTY2 (57-366) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LEFTY2
Gene ID: 7044
Uniprot ID: LFTY2_HUMAN
Immunogen Region: 57-366
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 57-366 of human LEFTY2 (NP_003231.2).
Immunogen Sequence: VRAQYVVLLRRSHGDRSRGK RFSQSFREVAGRFLASEAST HLLVFGMEQRLPPNSELVQA VLRLFQEPVPKAALHRHGRL SPRSAQARVTVEWLRVRDDG SNRTSLIDSRLVSVHESGWK AFDVTEAVNFWQQLSRPRQP LLLQVSVQREHLGPLASGAH KLVRFASQGAPAGLGEPQLE LHTLDLRDYGAQGDCDPEAP MTEGTRCCRQEMYIDLQGMK WAKNWVLEPPGFLAYECVG
Tissue Specificity Mesenchymal cells of the endometrial stroma.
Post Translational Modifications The processing of the protein may also occur at the second R-X-X-R site located at AA 132-135. Processing appears to be regulated in a cell-type specific manner.
Function Required for left-right (L-R) asymmetry determination of organ systems in mammals. May play a role in endometrial bleeding.
Protein Name Left-Right Determination Factor 2
Endometrial Bleeding-Associated Factor
Left-Right Determination Factor A
Protein Lefty-2
Protein Lefty-A
Transforming Growth Factor Beta-4
Tgf-Beta-4
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-1181150
Reactome: R-HSA-1433617
Cellular Localisation Secreted
Alternative Antibody Names Anti-Left-Right Determination Factor 2 antibody
Anti-Endometrial Bleeding-Associated Factor antibody
Anti-Left-Right Determination Factor A antibody
Anti-Protein Lefty-2 antibody
Anti-Protein Lefty-A antibody
Anti-Transforming Growth Factor Beta-4 antibody
Anti-Tgf-Beta-4 antibody
Anti-LEFTY2 antibody
Anti-EBAF antibody
Anti-LEFTA antibody
Anti-LEFTYA antibody
Anti-TGFB4 antibody
Anti-PSEC0024 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance