• Western blot analysis of MCF7, using LDHA antibody (STJ11101259) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts from wild type (WT) and LDHA knockout (KO) 293T cells, using [KO Validated] LDHA rabbit monoclonal antibody (STJ11101259) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] LDHA rabbit monoclonal antibody (STJ11101259) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using [KO Validated] LDHA rabbit monoclonal antibody (STJ11101259) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using [KO Validated] LDHA rabbit monoclonal antibody (STJ11101259) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-LDHA antibody (200-300) [S9MR] (STJ11101259)

SKU:
STJ11101259

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-LDHA (200-300) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LDHA
Gene ID: 3939
Uniprot ID: LDHA_HUMAN
Immunogen Region: 200-300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human LDHA (P00338).
Immunogen Sequence: VWSGMNVAGVSLKTLHPDLG TDKDKEQWKEVHKQVVESAY EVIKLKGYTSWAIGLSVADL AESIMKNLRRVHPVSTMIKG LYGIKDDVFLSVPCILGQNG I
Tissue Specificity Predominantly expressed in anaerobic tissues such as skeletal muscle and liver.
Post Translational Modifications ISGylated.
Function Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
Protein Name L-Lactate Dehydrogenase A Chain
Ldh-A
Cell Proliferation-Inducing Gene 19 Protein
Ldh Muscle Subunit
Ldh-M
Renal Carcinoma Antigen Ny-Ren-59
Database Links Reactome: R-HSA-70268
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-L-Lactate Dehydrogenase A Chain antibody
Anti-Ldh-A antibody
Anti-Cell Proliferation-Inducing Gene 19 Protein antibody
Anti-Ldh Muscle Subunit antibody
Anti-Ldh-M antibody
Anti-Renal Carcinoma Antigen Ny-Ren-59 antibody
Anti-LDHA antibody
Anti-PIG19 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance