• Immunofluorescence analysis of paraffin-embedded mouse bladder using Cytokeratin 13 (KRT13) (KRT13) Rabbit monoclonal antibody (STJ11101236) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded human cervix cancer using Cytokeratin 13 (KRT13) (KRT13) Rabbit monoclonal antibody (STJ11101236) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded rat bladder using Cytokeratin 13 (KRT13) (KRT13) Rabbit monoclonal antibody (STJ11101236) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Cytokeratin 13 (KRT13) in paraffin-embedded human lung squamous carcinoma tissue using Cytokeratin 13 (KRT13) (KRT13) Rabbit monoclonal antibody (STJ11101236) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Cytokeratin 13 (KRT13) (KRT13) Rabbit monoclonal antibody (STJ11101236) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-KRT13 antibody (100-200) [S6MR] (STJ11101236)

SKU:
STJ11101236

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Cytokeratin 13 (100-200) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT13
Gene ID: 3860
Uniprot ID: K1C13_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Cytokeratin 13 (KRT13) (P13646).
Immunogen Sequence: LTGNEKITMQNLNDRLASYL EKVRALEEANADLEVKIRDW HLKQSPASPERDYSPYYKTI EELRDKILTATIENNRVILE IDNARLAADDFRLKYENELA L
Tissue Specificity Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta.
Post Translational Modifications O-glycosylated.glycans consist of single N-acetylglucosamine residues.
Function Type 1 keratin (Probable). Maintains postnatal tongue mucosal cell homeostasis and tissue organization in response to mechanical stress, potentially via regulation of the G1/S phase cyclins CCNE1 and CCNE2.
Protein Name Keratin - Type I Cytoskeletal 13
Cytokeratin-13
Ck-13
Keratin-13
K13
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Alternative Antibody Names Anti-Keratin - Type I Cytoskeletal 13 antibody
Anti-Cytokeratin-13 antibody
Anti-Ck-13 antibody
Anti-Keratin-13 antibody
Anti-K13 antibody
Anti-KRT13 antibody

Information sourced from Uniprot.org

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