| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | KLHL3 |
| Gene ID: | 26249 |
| Uniprot ID: | KLHL3_HUMAN |
| Immunogen Region: | 1-110 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human KLHL3 (NP_059111.2). |
| Immunogen Sequence: | MEGESVKLSSQTLIQAGDDE KNQRTITVNPAHMGKAFKVM NELRSKQLLCDVMIVAEDVE IEAHRVVLAACSPYFCAMFT GDMSESKAKKIEIKDVDGQT LSKLIDYIYT |
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Phosphorylation at Ser-433 by PKA or PKC decreases the interaction with WNK1 and WNK4, leading to inhibit their degradation by the BCR(KLHL3) complex. Phosphorylated at Ser-433 by PKC in response to angiotensin II signaling, decreasing ability to promote degradation of WNK1 and WNK4, leading to activation of Na-Cl cotransporter SLC12A3/NCC. Phosphorylation at Ser-433 is increased by insulin. Dephosphorylated at Ser-433 by calcineurin PPP3CA, promoting degradation of WNK1 and WNK4. |
| Function | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of ion transport in the distal nephron. The BCR(KLHL3) complex acts by mediating ubiquitination and degradation of WNK1 and WNK4, two activators of Na-Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells of kidney, thereby regulating NaCl reabsorption. The BCR(KLHL3) complex also mediates ubiquitination and degradation of WNK3. The BCR(KLHL3) complex also mediates ubiquitination of CLDN8, a tight-junction protein required for paracellular chloride transport in the kidney, leading to its degradation. |
| Protein Name | Kelch-Like Protein 3 |
| Database Links | Reactome: R-HSA-8951664 Reactome: R-HSA-983168 |
| Cellular Localisation | Cytoplasm Cytosol Cytoskeleton |
| Alternative Antibody Names | Anti-Kelch-Like Protein 3 antibody Anti-KLHL3 antibody Anti-KIAA1129 antibody |
Information sourced from Uniprot.org