• Immunofluorescence analysis of A549 cells using KLF3 antibody (STJ29275).
  • Western blot analysis of extracts of various cell lines, using KLF3 antibody (STJ29275) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 60s.

Anti-KLF3 antibody (1-240) (STJ29275)

SKU:
STJ29275

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KLF3 (1-240) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KLF3
Gene ID: 51274
Uniprot ID: KLF3_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human KLF3 (NP_057615.3).
Immunogen Sequence: MLMFDPVPVKQEAMDPVSVS YPSNYMESMKPNKYGVIYST PLPEKFFQTPEGLSHGIQME PVDLTVNKRSSPPSAGNSPS SLKFPSSHRRASPGLSMPSS SPPIKKYSPPSPGVQPFGVP LSMPPVMAAALSRHGIRSPG ILPVIQPVVVQPVPFMYTSH LQQPLMVSLSEEMENSSSSM QVPVIESYEKPISQKKIKIE PGIEPQRTDYYPEEMSPPLM NSVSPPQALLQENHPSVIV
Post Translational Modifications Sumoylated with SUMO1. Sumoylation is enhanced by PIAS1, PIAS2alpha and PIAS2beta, and PIAS4, but not by Pc2. Enhances transcriptional repression, but has no effect on DNA binding. Sumoylation on Lys-198 is the major site.
Function Binds to the CACCC box of erythroid cell-expressed genes. May play a role in hematopoiesis.
Protein Name Krueppel-Like Factor 3
Basic Krueppel-Like Factor
Caccc-Box-Binding Protein Bklf
Tef-2
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Krueppel-Like Factor 3 antibody
Anti-Basic Krueppel-Like Factor antibody
Anti-Caccc-Box-Binding Protein Bklf antibody
Anti-Tef-2 antibody
Anti-KLF3 antibody
Anti-BKLF antibody

Information sourced from Uniprot.org

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