• Western blot analysis of extracts of various cell lines, using IVL antibody (STJ110332) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
  • Western blot analysis of various lysates using IVL Rabbit polyclonal antibody (STJ110332) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-IVL antibody (416-585) (STJ110332)

SKU:
STJ110332

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IVL (416-585) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IVL
Gene ID: 3713
Uniprot ID: INVO_HUMAN
Immunogen Region: 416-585
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 416-585 of human IVL (NP_005538.2).
Immunogen Sequence: QERQVEHLEQQVGQLKHLEE QEGQLKHLEQQQGQLEVPEQ QVGQPKNLEQEEKQLELPEQ QEGQVKHLEKQEAQLELPEQ QVGQPKHLEQQEKHLEHPEQ QDGQLKHLEQQEGQLKDLEQ QKGQLEQPVFAPAPGQVQDI QPALPTKGEVLLPVEHQQQK QEVQWPPKHK
Tissue Specificity Keratinocytes of epidermis and other stratified squamous epithelia.
Post Translational Modifications Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.
Function Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
Protein Name Involucrin
Database Links Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Constituent Of The Scaffolding Of The Cornified Envelope
Alternative Antibody Names Anti-Involucrin antibody
Anti-IVL antibody

Information sourced from Uniprot.org

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