• Perform microwave antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol. Immunofluorescence analysis of paraffin-embedded Rat Brain using IP3R Rabbit polyclonal antibody (STJ11104361) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using IP3R Rabbit polyclonal antibody (STJ11104361) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) : A-431. Exposure time: 10s.

Anti-ITPR1 antibody (1812-1911) (STJ11104361)

SKU:
STJ11104361

Current Stock:
Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Inositol 1-4-5-Trisphosphate Receptor Type 1 (1812-1911) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:50-1:200
IP 0.5ug-4ug antibody for 200ug-400ug extracts of whole cells
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ITPR1
Gene ID: 3708
Uniprot ID: ITPR1_HUMAN
Immunogen Region: 1812-1911
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1812-1911 of human IP3R (NP_001365381).
Immunogen Sequence: ASNLVIDLIMNASSDRVFHE SILLAIALLEGGNTTIQHSF FCRLTEDKKSEKFFKVFYDR MKVAQQEIKATVTVNTSDLG NKKKDDEVDRDAPSRKKAKE
Tissue Specificity Widely expressed.
Post Translational Modifications Phosphorylated on tyrosine residues. Ubiquitination at multiple lysines targets ITPR1 for proteasomal degradation. Approximately 40% of the ITPR1-associated ubiquitin is monoubiquitin, and polyubiquitins are both 'Lys-48'- and 'Lys-63'-linked. Phosphorylated by cAMP kinase (PKA). Phosphorylation prevents the ligand-induced opening of the calcium channels. Phosphorylation by PKA increases the interaction with inositol 1,4,5-trisphosphate and decreases the interaction with AHCYL1. Palmitoylated by ZDHHC6 in immune cells, leading to regulation of ITPR1 stability and function.
Function Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate. Involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1. Plays a role in ER stress-induced apoptosis. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaM kinase II, eventually leading to the activation of downstream apoptosis pathways.
Protein Name Inositol 1 -4 -5-Trisphosphate Receptor Type 1
Ip3 Receptor Isoform 1
Ip3r 1
Insp3r1
Type 1 Inositol 1 -4 -5-Trisphosphate Receptor
Type 1 Insp3 Receptor
Database Links Reactome: R-HSA-112043
Reactome: R-HSA-114508
Reactome: R-HSA-139853
Reactome: R-HSA-1489509
Reactome: R-HSA-2029485
Reactome: R-HSA-2871809
Reactome: R-HSA-381676
Reactome: R-HSA-4086398
Reactome: R-HSA-418457
Reactome: R-HSA-422356
Reactome: R-HSA-5218921
Reactome: R-HSA-5578775
Reactome: R-HSA-5607763
Reactome: R-HSA-9664323
Reactome: R-HSA-983695
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Cytoplasmic Vesicle
Secretory Vesicle Membrane
Cytoplasm
Perinuclear Region
Endoplasmic Reticulum And Secretory Granules
Alternative Antibody Names Anti-Inositol 1 -4 -5-Trisphosphate Receptor Type 1 antibody
Anti-Ip3 Receptor Isoform 1 antibody
Anti-Ip3r 1 antibody
Anti-Insp3r1 antibody
Anti-Type 1 Inositol 1 -4 -5-Trisphosphate Receptor antibody
Anti-Type 1 Insp3 Receptor antibody
Anti-ITPR1 antibody
Anti-INSP3R1 antibody

Information sourced from Uniprot.org

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